!Series_title	"Inhibition of the neuromuscular acetylcholine receptor with atracurium activates FOXO/DAF-16-induced longevity (RNA-seq)"
!Series_geo_accession	"GSE149944"
!Series_status	"Public on Jul 06 2021"
!Series_submission_date	"May 06 2020"
!Series_last_update_date	"Oct 05 2021"
!Series_pubmed_id	"34227219"
!Series_summary	"Transcriptome-based drug screening is emerging as a powerful tool to identify geroprotective compounds to intervene in age-related disease. We hypothesized that, by mimicking the transcriptional signature of the highly conserved longevity intervention of FOXO3 (daf-16 in worms) overexpression, we could identify and repurpose compounds with similar downstream effects to increase longevity. Our in silico screen, utilizing the LINCS transcriptome database of genetic and compound interventions, identified several FDA-approved compounds that activate FOXO downstream targets in mammalian cells. These included the neuromuscular blocker atracurium, which also robustly extends both lifespan and healthspan in C. elegans. This longevity is dependent on both daf-16 signaling and inhibition of the neuromuscular acetylcholine receptor. Other neuromuscular blockers tubocurarine and pancuronium caused similar healthspan benefits. Together, these data demonstrate the capacity to mimic genetic lifespan interventions with drugs, and in doing so, reveal that the neuromuscular acetylcholine receptor regulates the highly conserved FOXO/DAF-16 longevity pathway."
!Series_overall_design	"4 samples each of C. elegans treated for 24 hours from larval stage 4 to day 1 of adulthood with 50 μM atracurium besylate or water vehicle controls"
!Series_type	"Expression profiling by high throughput sequencing"
!Series_contributor	"Rebecca,L,McIntyre"
!Series_contributor	"Georges,E,Janssens"
!Series_sample_id	"GSM4518505 GSM4518506 GSM4518507 GSM4518508 GSM4518509 GSM4518510 GSM4518511 GSM4518512 "
!Series_contact_name	"Georges,E.,Janssens"
!Series_contact_email	"g.e.janssens@amsterdamumc.nl"
!Series_contact_department	"Laboratory Genetic Metabolic Diseases"
!Series_contact_institute	"Amsterdam UMC"
!Series_contact_address	"Meibergdreef 9"
!Series_contact_city	"Amsterdam"
!Series_contact_zip/postal_code	"1105 AZ"
!Series_contact_country	"Netherlands"
!Series_supplementary_file	"ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE149nnn/GSE149944/suppl/GSE149944_processed_atracurium.csv.gz"
!Series_platform_id	"GPL22765"
!Series_platform_taxid	"6239"
!Series_sample_taxid	"6239"
!Series_relation	"BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA630672"
!Series_relation	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP260227"

!Sample_title	"079_CTRL"	"080_CTRL"	"081_CTRL"	"082_CTRL"	"083_ATRA"	"084_ATRA"	"085_ATRA"	"086_ATRA"
!Sample_geo_accession	"GSM4518505"	"GSM4518506"	"GSM4518507"	"GSM4518508"	"GSM4518509"	"GSM4518510"	"GSM4518511"	"GSM4518512"
!Sample_status	"Public on Jul 06 2021"	"Public on Jul 06 2021"	"Public on Jul 06 2021"	"Public on Jul 06 2021"	"Public on Jul 06 2021"	"Public on Jul 06 2021"	"Public on Jul 06 2021"	"Public on Jul 06 2021"
!Sample_submission_date	"May 06 2020"	"May 06 2020"	"May 06 2020"	"May 06 2020"	"May 06 2020"	"May 06 2020"	"May 06 2020"	"May 06 2020"
!Sample_last_update_date	"Jul 06 2021"	"Jul 06 2021"	"Jul 06 2021"	"Jul 06 2021"	"Jul 06 2021"	"Jul 06 2021"	"Jul 06 2021"	"Jul 06 2021"
!Sample_type	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"
!Sample_channel_count	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"
!Sample_source_name_ch1	"whole worm"	"whole worm"	"whole worm"	"whole worm"	"whole worm"	"whole worm"	"whole worm"	"whole worm"
!Sample_organism_ch1	"Caenorhabditis elegans"	"Caenorhabditis elegans"	"Caenorhabditis elegans"	"Caenorhabditis elegans"	"Caenorhabditis elegans"	"Caenorhabditis elegans"	"Caenorhabditis elegans"	"Caenorhabditis elegans"
!Sample_characteristics_ch1	"tissue: whole worm"	"tissue: whole worm"	"tissue: whole worm"	"tissue: whole worm"	"tissue: whole worm"	"tissue: whole worm"	"tissue: whole worm"	"tissue: whole worm"
!Sample_characteristics_ch1	"strain: N2"	"strain: N2"	"strain: N2"	"strain: N2"	"strain: N2"	"strain: N2"	"strain: N2"	"strain: N2"
!Sample_characteristics_ch1	"age: day 1 adult"	"age: day 1 adult"	"age: day 1 adult"	"age: day 1 adult"	"age: day 1 adult"	"age: day 1 adult"	"age: day 1 adult"	"age: day 1 adult"
!Sample_characteristics_ch1	"treatment: water vehicle"	"treatment: water vehicle"	"treatment: water vehicle"	"treatment: water vehicle"	"treatment: 50 μM atracurium"	"treatment: 50 μM atracurium"	"treatment: 50 μM atracurium"	"treatment: 50 μM atracurium"
!Sample_treatment_protocol_ch1	"~1000 worms were transferred to plates containing compounds, as well as 5-fluorouracil at larval stage 4 of development. They were treated with atracurium or water control for 24 hours before harvest. At time of harvest, worms were washed in buffer and water, then snap frozen in liquid nitrogen."	"~1000 worms were transferred to plates containing compounds, as well as 5-fluorouracil at larval stage 4 of development. They were treated with atracurium or water control for 24 hours before harvest. At time of harvest, worms were washed in buffer and water, then snap frozen in liquid nitrogen."	"~1000 worms were transferred to plates containing compounds, as well as 5-fluorouracil at larval stage 4 of development. They were treated with atracurium or water control for 24 hours before harvest. At time of harvest, worms were washed in buffer and water, then snap frozen in liquid nitrogen."	"~1000 worms were transferred to plates containing compounds, as well as 5-fluorouracil at larval stage 4 of development. They were treated with atracurium or water control for 24 hours before harvest. At time of harvest, worms were washed in buffer and water, then snap frozen in liquid nitrogen."	"~1000 worms were transferred to plates containing compounds, as well as 5-fluorouracil at larval stage 4 of development. They were treated with atracurium or water control for 24 hours before harvest. At time of harvest, worms were washed in buffer and water, then snap frozen in liquid nitrogen."	"~1000 worms were transferred to plates containing compounds, as well as 5-fluorouracil at larval stage 4 of development. They were treated with atracurium or water control for 24 hours before harvest. At time of harvest, worms were washed in buffer and water, then snap frozen in liquid nitrogen."	"~1000 worms were transferred to plates containing compounds, as well as 5-fluorouracil at larval stage 4 of development. They were treated with atracurium or water control for 24 hours before harvest. At time of harvest, worms were washed in buffer and water, then snap frozen in liquid nitrogen."	"~1000 worms were transferred to plates containing compounds, as well as 5-fluorouracil at larval stage 4 of development. They were treated with atracurium or water control for 24 hours before harvest. At time of harvest, worms were washed in buffer and water, then snap frozen in liquid nitrogen."
!Sample_growth_protocol_ch1	"Gravid adult worms were age-synchronized using alkaline hypochlorite treatment, and incubated in M9 buffer overnight. Larval stage 1 worms were seeded to NGM plates and grown to larval stage 4."	"Gravid adult worms were age-synchronized using alkaline hypochlorite treatment, and incubated in M9 buffer overnight. Larval stage 1 worms were seeded to NGM plates and grown to larval stage 4."	"Gravid adult worms were age-synchronized using alkaline hypochlorite treatment, and incubated in M9 buffer overnight. Larval stage 1 worms were seeded to NGM plates and grown to larval stage 4."	"Gravid adult worms were age-synchronized using alkaline hypochlorite treatment, and incubated in M9 buffer overnight. Larval stage 1 worms were seeded to NGM plates and grown to larval stage 4."	"Gravid adult worms were age-synchronized using alkaline hypochlorite treatment, and incubated in M9 buffer overnight. Larval stage 1 worms were seeded to NGM plates and grown to larval stage 4."	"Gravid adult worms were age-synchronized using alkaline hypochlorite treatment, and incubated in M9 buffer overnight. Larval stage 1 worms were seeded to NGM plates and grown to larval stage 4."	"Gravid adult worms were age-synchronized using alkaline hypochlorite treatment, and incubated in M9 buffer overnight. Larval stage 1 worms were seeded to NGM plates and grown to larval stage 4."	"Gravid adult worms were age-synchronized using alkaline hypochlorite treatment, and incubated in M9 buffer overnight. Larval stage 1 worms were seeded to NGM plates and grown to larval stage 4."
!Sample_molecule_ch1	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"
!Sample_extract_protocol_ch1	"For isolation of total mRNA, whole worms were homogenized with a 5 mm steel bead using a TissueLyser II (QIAGEN) for 5 min at frequency of 30 times/second. RNA was extracted according to the instructions of the RNaesy Mini Kit (QIAGEN). Contaminating genomic DNA was removed using RNase-Free DNase (QIAGEN). RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific; Breda, The Netherlands) and stored at -80°C until use."	"For isolation of total mRNA, whole worms were homogenized with a 5 mm steel bead using a TissueLyser II (QIAGEN) for 5 min at frequency of 30 times/second. RNA was extracted according to the instructions of the RNaesy Mini Kit (QIAGEN). Contaminating genomic DNA was removed using RNase-Free DNase (QIAGEN). RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific; Breda, The Netherlands) and stored at -80°C until use."	"For isolation of total mRNA, whole worms were homogenized with a 5 mm steel bead using a TissueLyser II (QIAGEN) for 5 min at frequency of 30 times/second. RNA was extracted according to the instructions of the RNaesy Mini Kit (QIAGEN). Contaminating genomic DNA was removed using RNase-Free DNase (QIAGEN). RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific; Breda, The Netherlands) and stored at -80°C until use."	"For isolation of total mRNA, whole worms were homogenized with a 5 mm steel bead using a TissueLyser II (QIAGEN) for 5 min at frequency of 30 times/second. RNA was extracted according to the instructions of the RNaesy Mini Kit (QIAGEN). Contaminating genomic DNA was removed using RNase-Free DNase (QIAGEN). RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific; Breda, The Netherlands) and stored at -80°C until use."	"For isolation of total mRNA, whole worms were homogenized with a 5 mm steel bead using a TissueLyser II (QIAGEN) for 5 min at frequency of 30 times/second. RNA was extracted according to the instructions of the RNaesy Mini Kit (QIAGEN). Contaminating genomic DNA was removed using RNase-Free DNase (QIAGEN). RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific; Breda, The Netherlands) and stored at -80°C until use."	"For isolation of total mRNA, whole worms were homogenized with a 5 mm steel bead using a TissueLyser II (QIAGEN) for 5 min at frequency of 30 times/second. RNA was extracted according to the instructions of the RNaesy Mini Kit (QIAGEN). Contaminating genomic DNA was removed using RNase-Free DNase (QIAGEN). RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific; Breda, The Netherlands) and stored at -80°C until use."	"For isolation of total mRNA, whole worms were homogenized with a 5 mm steel bead using a TissueLyser II (QIAGEN) for 5 min at frequency of 30 times/second. RNA was extracted according to the instructions of the RNaesy Mini Kit (QIAGEN). Contaminating genomic DNA was removed using RNase-Free DNase (QIAGEN). RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific; Breda, The Netherlands) and stored at -80°C until use."	"For isolation of total mRNA, whole worms were homogenized with a 5 mm steel bead using a TissueLyser II (QIAGEN) for 5 min at frequency of 30 times/second. RNA was extracted according to the instructions of the RNaesy Mini Kit (QIAGEN). Contaminating genomic DNA was removed using RNase-Free DNase (QIAGEN). RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific; Breda, The Netherlands) and stored at -80°C until use."
!Sample_extract_protocol_ch1	"RNA libraries were prepared and sequenced with the Illumina platform by Genome Scan (Leiden, The Netherlands). The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the sample(s). The sample preparation was performed according to the protocol ""NEBNext Ultra II Directional RNA Library Prep Kit for Illumina"" (NEB #E7760S/L). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with the Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp). Clustering and DNA sequencing using the NovaSeq6000 was performed according to manufacturer's protocols. A concentration of 1.1 nM of DNA was used. NovaSeq control software NCS v1.6 was used."	"RNA libraries were prepared and sequenced with the Illumina platform by Genome Scan (Leiden, The Netherlands). The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the sample(s). The sample preparation was performed according to the protocol ""NEBNext Ultra II Directional RNA Library Prep Kit for Illumina"" (NEB #E7760S/L). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with the Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp). Clustering and DNA sequencing using the NovaSeq6000 was performed according to manufacturer's protocols. A concentration of 1.1 nM of DNA was used. NovaSeq control software NCS v1.6 was used."	"RNA libraries were prepared and sequenced with the Illumina platform by Genome Scan (Leiden, The Netherlands). The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the sample(s). The sample preparation was performed according to the protocol ""NEBNext Ultra II Directional RNA Library Prep Kit for Illumina"" (NEB #E7760S/L). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with the Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp). Clustering and DNA sequencing using the NovaSeq6000 was performed according to manufacturer's protocols. A concentration of 1.1 nM of DNA was used. NovaSeq control software NCS v1.6 was used."	"RNA libraries were prepared and sequenced with the Illumina platform by Genome Scan (Leiden, The Netherlands). The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the sample(s). The sample preparation was performed according to the protocol ""NEBNext Ultra II Directional RNA Library Prep Kit for Illumina"" (NEB #E7760S/L). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with the Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp). Clustering and DNA sequencing using the NovaSeq6000 was performed according to manufacturer's protocols. A concentration of 1.1 nM of DNA was used. NovaSeq control software NCS v1.6 was used."	"RNA libraries were prepared and sequenced with the Illumina platform by Genome Scan (Leiden, The Netherlands). The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the sample(s). The sample preparation was performed according to the protocol ""NEBNext Ultra II Directional RNA Library Prep Kit for Illumina"" (NEB #E7760S/L). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with the Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp). Clustering and DNA sequencing using the NovaSeq6000 was performed according to manufacturer's protocols. A concentration of 1.1 nM of DNA was used. NovaSeq control software NCS v1.6 was used."	"RNA libraries were prepared and sequenced with the Illumina platform by Genome Scan (Leiden, The Netherlands). The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the sample(s). The sample preparation was performed according to the protocol ""NEBNext Ultra II Directional RNA Library Prep Kit for Illumina"" (NEB #E7760S/L). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with the Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp). Clustering and DNA sequencing using the NovaSeq6000 was performed according to manufacturer's protocols. A concentration of 1.1 nM of DNA was used. NovaSeq control software NCS v1.6 was used."	"RNA libraries were prepared and sequenced with the Illumina platform by Genome Scan (Leiden, The Netherlands). The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the sample(s). The sample preparation was performed according to the protocol ""NEBNext Ultra II Directional RNA Library Prep Kit for Illumina"" (NEB #E7760S/L). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with the Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp). Clustering and DNA sequencing using the NovaSeq6000 was performed according to manufacturer's protocols. A concentration of 1.1 nM of DNA was used. NovaSeq control software NCS v1.6 was used."	"RNA libraries were prepared and sequenced with the Illumina platform by Genome Scan (Leiden, The Netherlands). The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the sample(s). The sample preparation was performed according to the protocol ""NEBNext Ultra II Directional RNA Library Prep Kit for Illumina"" (NEB #E7760S/L). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with the Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp). Clustering and DNA sequencing using the NovaSeq6000 was performed according to manufacturer's protocols. A concentration of 1.1 nM of DNA was used. NovaSeq control software NCS v1.6 was used."
!Sample_taxid_ch1	"6239"	"6239"	"6239"	"6239"	"6239"	"6239"	"6239"	"6239"
!Sample_description	"H2O_1"	"H2O_2"	"H2O_3"	"H2O_4"	"Atra_1"	"Atra_2"	"Atra_3"	"Atra_4"
!Sample_data_processing	"Reads were subjected to quality control FastQC, trimmed using Trimmomatic v0.32 and aligned to the C. elegans genome obtained from Ensembl (wbcel235.v91), using HISAT2 v2.1.0"	"Reads were subjected to quality control FastQC, trimmed using Trimmomatic v0.32 and aligned to the C. elegans genome obtained from Ensembl (wbcel235.v91), using HISAT2 v2.1.0"	"Reads were subjected to quality control FastQC, trimmed using Trimmomatic v0.32 and aligned to the C. elegans genome obtained from Ensembl (wbcel235.v91), using HISAT2 v2.1.0"	"Reads were subjected to quality control FastQC, trimmed using Trimmomatic v0.32 and aligned to the C. elegans genome obtained from Ensembl (wbcel235.v91), using HISAT2 v2.1.0"	"Reads were subjected to quality control FastQC, trimmed using Trimmomatic v0.32 and aligned to the C. elegans genome obtained from Ensembl (wbcel235.v91), using HISAT2 v2.1.0"	"Reads were subjected to quality control FastQC, trimmed using Trimmomatic v0.32 and aligned to the C. elegans genome obtained from Ensembl (wbcel235.v91), using HISAT2 v2.1.0"	"Reads were subjected to quality control FastQC, trimmed using Trimmomatic v0.32 and aligned to the C. elegans genome obtained from Ensembl (wbcel235.v91), using HISAT2 v2.1.0"	"Reads were subjected to quality control FastQC, trimmed using Trimmomatic v0.32 and aligned to the C. elegans genome obtained from Ensembl (wbcel235.v91), using HISAT2 v2.1.0"
!Sample_data_processing	"Counts were obtained using HTSeq (v0.11.0, default parameters) using the corresponding GTF taking into account the directions of the reads. Statistical analyses were performed using the edgeR v3.26.8 and limma/voom v 3.40.6 R packages"	"Counts were obtained using HTSeq (v0.11.0, default parameters) using the corresponding GTF taking into account the directions of the reads. Statistical analyses were performed using the edgeR v3.26.8 and limma/voom v 3.40.6 R packages"	"Counts were obtained using HTSeq (v0.11.0, default parameters) using the corresponding GTF taking into account the directions of the reads. Statistical analyses were performed using the edgeR v3.26.8 and limma/voom v 3.40.6 R packages"	"Counts were obtained using HTSeq (v0.11.0, default parameters) using the corresponding GTF taking into account the directions of the reads. Statistical analyses were performed using the edgeR v3.26.8 and limma/voom v 3.40.6 R packages"	"Counts were obtained using HTSeq (v0.11.0, default parameters) using the corresponding GTF taking into account the directions of the reads. Statistical analyses were performed using the edgeR v3.26.8 and limma/voom v 3.40.6 R packages"	"Counts were obtained using HTSeq (v0.11.0, default parameters) using the corresponding GTF taking into account the directions of the reads. Statistical analyses were performed using the edgeR v3.26.8 and limma/voom v 3.40.6 R packages"	"Counts were obtained using HTSeq (v0.11.0, default parameters) using the corresponding GTF taking into account the directions of the reads. Statistical analyses were performed using the edgeR v3.26.8 and limma/voom v 3.40.6 R packages"	"Counts were obtained using HTSeq (v0.11.0, default parameters) using the corresponding GTF taking into account the directions of the reads. Statistical analyses were performed using the edgeR v3.26.8 and limma/voom v 3.40.6 R packages"
!Sample_data_processing	"All genes with more than 2 counts in at least 3 of the samples were kept. Count data were transformed to log2-counts per million (logCPM), normalized by applying the trimmed mean of M-values method and precision weighted using voom."	"All genes with more than 2 counts in at least 3 of the samples were kept. Count data were transformed to log2-counts per million (logCPM), normalized by applying the trimmed mean of M-values method and precision weighted using voom."	"All genes with more than 2 counts in at least 3 of the samples were kept. Count data were transformed to log2-counts per million (logCPM), normalized by applying the trimmed mean of M-values method and precision weighted using voom."	"All genes with more than 2 counts in at least 3 of the samples were kept. Count data were transformed to log2-counts per million (logCPM), normalized by applying the trimmed mean of M-values method and precision weighted using voom."	"All genes with more than 2 counts in at least 3 of the samples were kept. Count data were transformed to log2-counts per million (logCPM), normalized by applying the trimmed mean of M-values method and precision weighted using voom."	"All genes with more than 2 counts in at least 3 of the samples were kept. Count data were transformed to log2-counts per million (logCPM), normalized by applying the trimmed mean of M-values method and precision weighted using voom."	"All genes with more than 2 counts in at least 3 of the samples were kept. Count data were transformed to log2-counts per million (logCPM), normalized by applying the trimmed mean of M-values method and precision weighted using voom."	"All genes with more than 2 counts in at least 3 of the samples were kept. Count data were transformed to log2-counts per million (logCPM), normalized by applying the trimmed mean of M-values method and precision weighted using voom."
!Sample_data_processing	"Genome_build: wbcel235.v91"	"Genome_build: wbcel235.v91"	"Genome_build: wbcel235.v91"	"Genome_build: wbcel235.v91"	"Genome_build: wbcel235.v91"	"Genome_build: wbcel235.v91"	"Genome_build: wbcel235.v91"	"Genome_build: wbcel235.v91"
!Sample_data_processing	"Supplementary_files_format_and_content: csv file of CMPs (counts per million) for RNAseq data mapped to gene level for each sample"	"Supplementary_files_format_and_content: csv file of CMPs (counts per million) for RNAseq data mapped to gene level for each sample"	"Supplementary_files_format_and_content: csv file of CMPs (counts per million) for RNAseq data mapped to gene level for each sample"	"Supplementary_files_format_and_content: csv file of CMPs (counts per million) for RNAseq data mapped to gene level for each sample"	"Supplementary_files_format_and_content: csv file of CMPs (counts per million) for RNAseq data mapped to gene level for each sample"	"Supplementary_files_format_and_content: csv file of CMPs (counts per million) for RNAseq data mapped to gene level for each sample"	"Supplementary_files_format_and_content: csv file of CMPs (counts per million) for RNAseq data mapped to gene level for each sample"	"Supplementary_files_format_and_content: csv file of CMPs (counts per million) for RNAseq data mapped to gene level for each sample"
!Sample_platform_id	"GPL22765"	"GPL22765"	"GPL22765"	"GPL22765"	"GPL22765"	"GPL22765"	"GPL22765"	"GPL22765"
!Sample_contact_name	"Georges,E.,Janssens"	"Georges,E.,Janssens"	"Georges,E.,Janssens"	"Georges,E.,Janssens"	"Georges,E.,Janssens"	"Georges,E.,Janssens"	"Georges,E.,Janssens"	"Georges,E.,Janssens"
!Sample_contact_email	"g.e.janssens@amsterdamumc.nl"	"g.e.janssens@amsterdamumc.nl"	"g.e.janssens@amsterdamumc.nl"	"g.e.janssens@amsterdamumc.nl"	"g.e.janssens@amsterdamumc.nl"	"g.e.janssens@amsterdamumc.nl"	"g.e.janssens@amsterdamumc.nl"	"g.e.janssens@amsterdamumc.nl"
!Sample_contact_department	"Laboratory Genetic Metabolic Diseases"	"Laboratory Genetic Metabolic Diseases"	"Laboratory Genetic Metabolic Diseases"	"Laboratory Genetic Metabolic Diseases"	"Laboratory Genetic Metabolic Diseases"	"Laboratory Genetic Metabolic Diseases"	"Laboratory Genetic Metabolic Diseases"	"Laboratory Genetic Metabolic Diseases"
!Sample_contact_institute	"Amsterdam UMC"	"Amsterdam UMC"	"Amsterdam UMC"	"Amsterdam UMC"	"Amsterdam UMC"	"Amsterdam UMC"	"Amsterdam UMC"	"Amsterdam UMC"
!Sample_contact_address	"Meibergdreef 9"	"Meibergdreef 9"	"Meibergdreef 9"	"Meibergdreef 9"	"Meibergdreef 9"	"Meibergdreef 9"	"Meibergdreef 9"	"Meibergdreef 9"
!Sample_contact_city	"Amsterdam"	"Amsterdam"	"Amsterdam"	"Amsterdam"	"Amsterdam"	"Amsterdam"	"Amsterdam"	"Amsterdam"
!Sample_contact_zip/postal_code	"1105 AZ"	"1105 AZ"	"1105 AZ"	"1105 AZ"	"1105 AZ"	"1105 AZ"	"1105 AZ"	"1105 AZ"
!Sample_contact_country	"Netherlands"	"Netherlands"	"Netherlands"	"Netherlands"	"Netherlands"	"Netherlands"	"Netherlands"	"Netherlands"
!Sample_data_row_count	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"
!Sample_instrument_model	"Illumina HiSeq 4000"	"Illumina HiSeq 4000"	"Illumina HiSeq 4000"	"Illumina HiSeq 4000"	"Illumina HiSeq 4000"	"Illumina HiSeq 4000"	"Illumina HiSeq 4000"	"Illumina HiSeq 4000"
!Sample_library_selection	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"
!Sample_library_source	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"
!Sample_library_strategy	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"
!Sample_relation	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN14841266"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN14841265"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN14841264"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN14841263"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN14841262"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN14841261"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN14841260"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN14841259"
!Sample_relation	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8264329"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8264322"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8264323"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8264324"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8264325"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8264326"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8264327"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8264328"
!Sample_supplementary_file_1	"NONE"	"NONE"	"NONE"	"NONE"	"NONE"	"NONE"	"NONE"	"NONE"
!series_matrix_table_begin
"ID_REF"	"GSM4518505"	"GSM4518506"	"GSM4518507"	"GSM4518508"	"GSM4518509"	"GSM4518510"	"GSM4518511"	"GSM4518512"
!series_matrix_table_end
