!Series_title	"Transgenerational transmission of aspartame induced anxiety and changes in glutamate-GABA signaling and gene expression in the amygdala"
!Series_geo_accession	"GSE210561"
!Series_status	"Public on Oct 12 2022"
!Series_submission_date	"Aug 04 2022"
!Series_last_update_date	"Dec 12 2022"
!Series_pubmed_id	"36459641"
!Series_summary	"We show that daily exposure of mice to aspartame, an artificial sweetener found in over 5,000 diet foods and drinks at doses equivalent to only 15% of the FDA recommended maximum daily intake for humans produces anxiety. The anxiety is alleviated by diazepam, a drug used in the treatment of generalized anxiety disorder. We completed RNA sequencing of the amygdala of male mice that were exposed to aspartame or plain drinking water (control), as this brain region regulates anxiety and fear. The aspartame exposure produces changes in the expression of genes regulating excitation-inhibition balance in the amygdala. The anxiety, its response to diazepam and the changes in amygdala gene expression are not limited to the aspartame-exposed individuals but also appear in up to two generations descending from the aspartame-exposed males. Thus, aspartame’s neurobehavioral effects are not limited to those who consume it but also impact generations of descendants, a finding that highlights the need for a re-evaluation of current policies on artificial sweetener use."
!Series_overall_design	"Amygdala mRNA from adult C57BL/6 males exposed to either 0.03% aspartame or plain drinking water were analyzed by RNA sequencing of 4 biological replicates using an Illumina NovaSeq 6000"
!Series_type	"Expression profiling by high throughput sequencing"
!Series_contributor	"Sara,K,Jones"
!Series_contributor	"Deirdre,M,McCarthy"
!Series_contributor	"Cynthia,,Vied"
!Series_contributor	"Pradeep,G,Bhide"
!Series_sample_id	"GSM6432570 GSM6432571 GSM6432572 GSM6432573 GSM6432574 GSM6432575 GSM6432576 GSM6432577 "
!Series_contact_name	"Cynthia,,Vied"
!Series_contact_email	"cynthia.vied@med.fsu.edu"
!Series_contact_department	"Translational Science Laboratory"
!Series_contact_institute	"Florida State University"
!Series_contact_address	"1115 W. Call St"
!Series_contact_city	"Tallahassee"
!Series_contact_state	"Florida"
!Series_contact_zip/postal_code	"32306"
!Series_contact_country	"USA"
!Series_supplementary_file	"ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE210nnn/GSE210561/suppl/GSE210561_rlog_Amygdala.xlsx"
!Series_platform_id	"GPL24247"
!Series_platform_taxid	"10090"
!Series_sample_taxid	"10090"
!Series_relation	"BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA866165"

!Sample_title	"Water 1"	"Water 2"	"Water 3"	"Water 4"	"Aspartame 1"	"Aspartame 2"	"Aspartame 3"	"Aspartame 4"
!Sample_geo_accession	"GSM6432570"	"GSM6432571"	"GSM6432572"	"GSM6432573"	"GSM6432574"	"GSM6432575"	"GSM6432576"	"GSM6432577"
!Sample_status	"Public on Oct 12 2022"	"Public on Oct 12 2022"	"Public on Oct 12 2022"	"Public on Oct 12 2022"	"Public on Oct 12 2022"	"Public on Oct 12 2022"	"Public on Oct 12 2022"	"Public on Oct 12 2022"
!Sample_submission_date	"Aug 04 2022"	"Aug 04 2022"	"Aug 04 2022"	"Aug 04 2022"	"Aug 04 2022"	"Aug 04 2022"	"Aug 04 2022"	"Aug 04 2022"
!Sample_last_update_date	"Oct 12 2022"	"Oct 12 2022"	"Oct 12 2022"	"Oct 12 2022"	"Oct 12 2022"	"Oct 12 2022"	"Oct 12 2022"	"Oct 12 2022"
!Sample_type	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"
!Sample_channel_count	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"
!Sample_source_name_ch1	"Amygdala"	"Amygdala"	"Amygdala"	"Amygdala"	"Amygdala"	"Amygdala"	"Amygdala"	"Amygdala"
!Sample_organism_ch1	"Mus musculus"	"Mus musculus"	"Mus musculus"	"Mus musculus"	"Mus musculus"	"Mus musculus"	"Mus musculus"	"Mus musculus"
!Sample_characteristics_ch1	"strain: C57BL/6"	"strain: C57BL/6"	"strain: C57BL/6"	"strain: C57BL/6"	"strain: C57BL/6"	"strain: C57BL/6"	"strain: C57BL/6"	"strain: C57BL/6"
!Sample_characteristics_ch1	"Sex: Male"	"Sex: Male"	"Sex: Male"	"Sex: Male"	"Sex: Male"	"Sex: Male"	"Sex: Male"	"Sex: Male"
!Sample_characteristics_ch1	"age: 187 Days"	"age: 189 Days"	"age: 187 Days"	"age: 189 Days"	"age: 188 Days"	"age: 189 Days"	"age: 187 Days"	"age: 187 Days"
!Sample_characteristics_ch1	"tissue: Amygdala"	"tissue: Amygdala"	"tissue: Amygdala"	"tissue: Amygdala"	"tissue: Amygdala"	"tissue: Amygdala"	"tissue: Amygdala"	"tissue: Amygdala"
!Sample_characteristics_ch1	"agent: water"	"agent: water"	"agent: water"	"agent: water"	"agent: Aspartame"	"agent: Aspartame"	"agent: Aspartame"	"agent: Aspartame"
!Sample_treatment_protocol_ch1	"Mice were provided either plain drinking water or water containing 0.03% aspartame (Sigma Aldrich, # A5139) for 18 weeks."	"Mice were provided either plain drinking water or water containing 0.03% aspartame (Sigma Aldrich, # A5139) for 18 weeks."	"Mice were provided either plain drinking water or water containing 0.03% aspartame (Sigma Aldrich, # A5139) for 18 weeks."	"Mice were provided either plain drinking water or water containing 0.03% aspartame (Sigma Aldrich, # A5139) for 18 weeks."	"Mice were provided either plain drinking water or water containing 0.03% aspartame (Sigma Aldrich, # A5139) for 18 weeks."	"Mice were provided either plain drinking water or water containing 0.03% aspartame (Sigma Aldrich, # A5139) for 18 weeks."	"Mice were provided either plain drinking water or water containing 0.03% aspartame (Sigma Aldrich, # A5139) for 18 weeks."	"Mice were provided either plain drinking water or water containing 0.03% aspartame (Sigma Aldrich, # A5139) for 18 weeks."
!Sample_growth_protocol_ch1	"8 week old male mice were purchased from Charles River Laboratory (Wilmington,MA). Animals were kept on a 12 hour light-dark cycle. Food and water were provided ad libitum. Animals were group housed."	"8 week old male mice were purchased from Charles River Laboratory (Wilmington,MA). Animals were kept on a 12 hour light-dark cycle. Food and water were provided ad libitum. Animals were group housed."	"8 week old male mice were purchased from Charles River Laboratory (Wilmington,MA). Animals were kept on a 12 hour light-dark cycle. Food and water were provided ad libitum. Animals were group housed."	"8 week old male mice were purchased from Charles River Laboratory (Wilmington,MA). Animals were kept on a 12 hour light-dark cycle. Food and water were provided ad libitum. Animals were group housed."	"8 week old male mice were purchased from Charles River Laboratory (Wilmington,MA). Animals were kept on a 12 hour light-dark cycle. Food and water were provided ad libitum. Animals were group housed."	"8 week old male mice were purchased from Charles River Laboratory (Wilmington,MA). Animals were kept on a 12 hour light-dark cycle. Food and water were provided ad libitum. Animals were group housed."	"8 week old male mice were purchased from Charles River Laboratory (Wilmington,MA). Animals were kept on a 12 hour light-dark cycle. Food and water were provided ad libitum. Animals were group housed."	"8 week old male mice were purchased from Charles River Laboratory (Wilmington,MA). Animals were kept on a 12 hour light-dark cycle. Food and water were provided ad libitum. Animals were group housed."
!Sample_molecule_ch1	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"
!Sample_extract_protocol_ch1	"Mice were anesthetized (Isoflurane, Pivetal, Liberty, MO), decapitated, and the amygdala was collected by microdissection. Samples were snap-frozen in liquid nitrogen.and stored at -80 degrees. Total RNA was extracted from the amygdala from each animal using  a RNeasy mini kit (Qiagen, 74104) according to manufacturer’s directions."	"Mice were anesthetized (Isoflurane, Pivetal, Liberty, MO), decapitated, and the amygdala was collected by microdissection. Samples were snap-frozen in liquid nitrogen.and stored at -80 degrees. Total RNA was extracted from the amygdala from each animal using  a RNeasy mini kit (Qiagen, 74104) according to manufacturer’s directions."	"Mice were anesthetized (Isoflurane, Pivetal, Liberty, MO), decapitated, and the amygdala was collected by microdissection. Samples were snap-frozen in liquid nitrogen.and stored at -80 degrees. Total RNA was extracted from the amygdala from each animal using  a RNeasy mini kit (Qiagen, 74104) according to manufacturer’s directions."	"Mice were anesthetized (Isoflurane, Pivetal, Liberty, MO), decapitated, and the amygdala was collected by microdissection. Samples were snap-frozen in liquid nitrogen.and stored at -80 degrees. Total RNA was extracted from the amygdala from each animal using  a RNeasy mini kit (Qiagen, 74104) according to manufacturer’s directions."	"Mice were anesthetized (Isoflurane, Pivetal, Liberty, MO), decapitated, and the amygdala was collected by microdissection. Samples were snap-frozen in liquid nitrogen.and stored at -80 degrees. Total RNA was extracted from the amygdala from each animal using  a RNeasy mini kit (Qiagen, 74104) according to manufacturer’s directions."	"Mice were anesthetized (Isoflurane, Pivetal, Liberty, MO), decapitated, and the amygdala was collected by microdissection. Samples were snap-frozen in liquid nitrogen.and stored at -80 degrees. Total RNA was extracted from the amygdala from each animal using  a RNeasy mini kit (Qiagen, 74104) according to manufacturer’s directions."	"Mice were anesthetized (Isoflurane, Pivetal, Liberty, MO), decapitated, and the amygdala was collected by microdissection. Samples were snap-frozen in liquid nitrogen.and stored at -80 degrees. Total RNA was extracted from the amygdala from each animal using  a RNeasy mini kit (Qiagen, 74104) according to manufacturer’s directions."	"Mice were anesthetized (Isoflurane, Pivetal, Liberty, MO), decapitated, and the amygdala was collected by microdissection. Samples were snap-frozen in liquid nitrogen.and stored at -80 degrees. Total RNA was extracted from the amygdala from each animal using  a RNeasy mini kit (Qiagen, 74104) according to manufacturer’s directions."
!Sample_extract_protocol_ch1	"A cDNA library was prepared for each sample using the Illumina TruSeq stranded mRNA library kit. Libraries were barcoded for multiplexing with IDT for Illumina 8 nucleotide i7 and i5 unique dual indexes. Each multiplexed library was sequenced on an Illumina NovaSeq 6000 as a 150 base pair paired-end sequencing run. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Bioanalyzer high sensitivity DNA chip (Agilent Technologies). The pooled sample was sequenced, with paired-end, 150 base reads on an Illumina NovaSeq 6000 located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data."	"A cDNA library was prepared for each sample using the Illumina TruSeq stranded mRNA library kit. Libraries were barcoded for multiplexing with IDT for Illumina 8 nucleotide i7 and i5 unique dual indexes. Each multiplexed library was sequenced on an Illumina NovaSeq 6000 as a 150 base pair paired-end sequencing run. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Bioanalyzer high sensitivity DNA chip (Agilent Technologies). The pooled sample was sequenced, with paired-end, 150 base reads on an Illumina NovaSeq 6000 located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data."	"A cDNA library was prepared for each sample using the Illumina TruSeq stranded mRNA library kit. Libraries were barcoded for multiplexing with IDT for Illumina 8 nucleotide i7 and i5 unique dual indexes. Each multiplexed library was sequenced on an Illumina NovaSeq 6000 as a 150 base pair paired-end sequencing run. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Bioanalyzer high sensitivity DNA chip (Agilent Technologies). The pooled sample was sequenced, with paired-end, 150 base reads on an Illumina NovaSeq 6000 located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data."	"A cDNA library was prepared for each sample using the Illumina TruSeq stranded mRNA library kit. Libraries were barcoded for multiplexing with IDT for Illumina 8 nucleotide i7 and i5 unique dual indexes. Each multiplexed library was sequenced on an Illumina NovaSeq 6000 as a 150 base pair paired-end sequencing run. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Bioanalyzer high sensitivity DNA chip (Agilent Technologies). The pooled sample was sequenced, with paired-end, 150 base reads on an Illumina NovaSeq 6000 located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data."	"A cDNA library was prepared for each sample using the Illumina TruSeq stranded mRNA library kit. Libraries were barcoded for multiplexing with IDT for Illumina 8 nucleotide i7 and i5 unique dual indexes. Each multiplexed library was sequenced on an Illumina NovaSeq 6000 as a 150 base pair paired-end sequencing run. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Bioanalyzer high sensitivity DNA chip (Agilent Technologies). The pooled sample was sequenced, with paired-end, 150 base reads on an Illumina NovaSeq 6000 located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data."	"A cDNA library was prepared for each sample using the Illumina TruSeq stranded mRNA library kit. Libraries were barcoded for multiplexing with IDT for Illumina 8 nucleotide i7 and i5 unique dual indexes. Each multiplexed library was sequenced on an Illumina NovaSeq 6000 as a 150 base pair paired-end sequencing run. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Bioanalyzer high sensitivity DNA chip (Agilent Technologies). The pooled sample was sequenced, with paired-end, 150 base reads on an Illumina NovaSeq 6000 located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data."	"A cDNA library was prepared for each sample using the Illumina TruSeq stranded mRNA library kit. Libraries were barcoded for multiplexing with IDT for Illumina 8 nucleotide i7 and i5 unique dual indexes. Each multiplexed library was sequenced on an Illumina NovaSeq 6000 as a 150 base pair paired-end sequencing run. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Bioanalyzer high sensitivity DNA chip (Agilent Technologies). The pooled sample was sequenced, with paired-end, 150 base reads on an Illumina NovaSeq 6000 located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data."	"A cDNA library was prepared for each sample using the Illumina TruSeq stranded mRNA library kit. Libraries were barcoded for multiplexing with IDT for Illumina 8 nucleotide i7 and i5 unique dual indexes. Each multiplexed library was sequenced on an Illumina NovaSeq 6000 as a 150 base pair paired-end sequencing run. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Bioanalyzer high sensitivity DNA chip (Agilent Technologies). The pooled sample was sequenced, with paired-end, 150 base reads on an Illumina NovaSeq 6000 located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data."
!Sample_taxid_ch1	"10090"	"10090"	"10090"	"10090"	"10090"	"10090"	"10090"	"10090"
!Sample_description	"Am_W1"	"Am_W2"	"Am_W3"	"Am_W4"	"Am_AL1"	"Am_AL2"	"Am_AL3"	"Am_AL4"
!Sample_data_processing	"Adapters were trimmed during demultiplexing by the core facility and trimmed reads were aligned to the genome using RNA-Seq Alignment version 1.1.1 (Illumina BaseSpace software application). The application uses STAR for alignment to the mouse genome (UCSC mm10) and Salmon was used to generate count tables."	"Adapters were trimmed during demultiplexing by the core facility and trimmed reads were aligned to the genome using RNA-Seq Alignment version 1.1.1 (Illumina BaseSpace software application). The application uses STAR for alignment to the mouse genome (UCSC mm10) and Salmon was used to generate count tables."	"Adapters were trimmed during demultiplexing by the core facility and trimmed reads were aligned to the genome using RNA-Seq Alignment version 1.1.1 (Illumina BaseSpace software application). The application uses STAR for alignment to the mouse genome (UCSC mm10) and Salmon was used to generate count tables."	"Adapters were trimmed during demultiplexing by the core facility and trimmed reads were aligned to the genome using RNA-Seq Alignment version 1.1.1 (Illumina BaseSpace software application). The application uses STAR for alignment to the mouse genome (UCSC mm10) and Salmon was used to generate count tables."	"Adapters were trimmed during demultiplexing by the core facility and trimmed reads were aligned to the genome using RNA-Seq Alignment version 1.1.1 (Illumina BaseSpace software application). The application uses STAR for alignment to the mouse genome (UCSC mm10) and Salmon was used to generate count tables."	"Adapters were trimmed during demultiplexing by the core facility and trimmed reads were aligned to the genome using RNA-Seq Alignment version 1.1.1 (Illumina BaseSpace software application). The application uses STAR for alignment to the mouse genome (UCSC mm10) and Salmon was used to generate count tables."	"Adapters were trimmed during demultiplexing by the core facility and trimmed reads were aligned to the genome using RNA-Seq Alignment version 1.1.1 (Illumina BaseSpace software application). The application uses STAR for alignment to the mouse genome (UCSC mm10) and Salmon was used to generate count tables."	"Adapters were trimmed during demultiplexing by the core facility and trimmed reads were aligned to the genome using RNA-Seq Alignment version 1.1.1 (Illumina BaseSpace software application). The application uses STAR for alignment to the mouse genome (UCSC mm10) and Salmon was used to generate count tables."
!Sample_data_processing	"Differential expression analysis of count tables was performed using the default settings of DESeq2 (Benjamini-Hochberg FDR correction)."	"Differential expression analysis of count tables was performed using the default settings of DESeq2 (Benjamini-Hochberg FDR correction)."	"Differential expression analysis of count tables was performed using the default settings of DESeq2 (Benjamini-Hochberg FDR correction)."	"Differential expression analysis of count tables was performed using the default settings of DESeq2 (Benjamini-Hochberg FDR correction)."	"Differential expression analysis of count tables was performed using the default settings of DESeq2 (Benjamini-Hochberg FDR correction)."	"Differential expression analysis of count tables was performed using the default settings of DESeq2 (Benjamini-Hochberg FDR correction)."	"Differential expression analysis of count tables was performed using the default settings of DESeq2 (Benjamini-Hochberg FDR correction)."	"Differential expression analysis of count tables was performed using the default settings of DESeq2 (Benjamini-Hochberg FDR correction)."
!Sample_data_processing	"Assembly: mm10 (GRCm38)"	"Assembly: mm10 (GRCm38)"	"Assembly: mm10 (GRCm38)"	"Assembly: mm10 (GRCm38)"	"Assembly: mm10 (GRCm38)"	"Assembly: mm10 (GRCm38)"	"Assembly: mm10 (GRCm38)"	"Assembly: mm10 (GRCm38)"
!Sample_data_processing	"Supplementary files format and content: Microsoft Excel Worksheet (.xlsx). Contains normalized counts generated by DESeq2."	"Supplementary files format and content: Microsoft Excel Worksheet (.xlsx). Contains normalized counts generated by DESeq2."	"Supplementary files format and content: Microsoft Excel Worksheet (.xlsx). Contains normalized counts generated by DESeq2."	"Supplementary files format and content: Microsoft Excel Worksheet (.xlsx). Contains normalized counts generated by DESeq2."	"Supplementary files format and content: Microsoft Excel Worksheet (.xlsx). Contains normalized counts generated by DESeq2."	"Supplementary files format and content: Microsoft Excel Worksheet (.xlsx). Contains normalized counts generated by DESeq2."	"Supplementary files format and content: Microsoft Excel Worksheet (.xlsx). Contains normalized counts generated by DESeq2."	"Supplementary files format and content: Microsoft Excel Worksheet (.xlsx). Contains normalized counts generated by DESeq2."
!Sample_platform_id	"GPL24247"	"GPL24247"	"GPL24247"	"GPL24247"	"GPL24247"	"GPL24247"	"GPL24247"	"GPL24247"
!Sample_contact_name	"Cynthia,,Vied"	"Cynthia,,Vied"	"Cynthia,,Vied"	"Cynthia,,Vied"	"Cynthia,,Vied"	"Cynthia,,Vied"	"Cynthia,,Vied"	"Cynthia,,Vied"
!Sample_contact_email	"cynthia.vied@med.fsu.edu"	"cynthia.vied@med.fsu.edu"	"cynthia.vied@med.fsu.edu"	"cynthia.vied@med.fsu.edu"	"cynthia.vied@med.fsu.edu"	"cynthia.vied@med.fsu.edu"	"cynthia.vied@med.fsu.edu"	"cynthia.vied@med.fsu.edu"
!Sample_contact_department	"Translational Science Laboratory"	"Translational Science Laboratory"	"Translational Science Laboratory"	"Translational Science Laboratory"	"Translational Science Laboratory"	"Translational Science Laboratory"	"Translational Science Laboratory"	"Translational Science Laboratory"
!Sample_contact_institute	"Florida State University"	"Florida State University"	"Florida State University"	"Florida State University"	"Florida State University"	"Florida State University"	"Florida State University"	"Florida State University"
!Sample_contact_address	"1115 W. Call St"	"1115 W. Call St"	"1115 W. Call St"	"1115 W. Call St"	"1115 W. Call St"	"1115 W. Call St"	"1115 W. Call St"	"1115 W. Call St"
!Sample_contact_city	"Tallahassee"	"Tallahassee"	"Tallahassee"	"Tallahassee"	"Tallahassee"	"Tallahassee"	"Tallahassee"	"Tallahassee"
!Sample_contact_state	"Florida"	"Florida"	"Florida"	"Florida"	"Florida"	"Florida"	"Florida"	"Florida"
!Sample_contact_zip/postal_code	"32306"	"32306"	"32306"	"32306"	"32306"	"32306"	"32306"	"32306"
!Sample_contact_country	"USA"	"USA"	"USA"	"USA"	"USA"	"USA"	"USA"	"USA"
!Sample_data_row_count	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"
!Sample_instrument_model	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"
!Sample_library_selection	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"
!Sample_library_source	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"
!Sample_library_strategy	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"
!Sample_relation	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN30154658"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN30154657"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN30154656"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN30154655"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN30154654"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN30154653"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN30154652"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN30154651"
!Sample_relation	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX16849749"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX16849750"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX16849751"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX16849752"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX16849753"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX16849754"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX16849755"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX16849756"
!Sample_supplementary_file_1	"NONE"	"NONE"	"NONE"	"NONE"	"NONE"	"NONE"	"NONE"	"NONE"
!series_matrix_table_begin
"ID_REF"	"GSM6432570"	"GSM6432571"	"GSM6432572"	"GSM6432573"	"GSM6432574"	"GSM6432575"	"GSM6432576"	"GSM6432577"
!series_matrix_table_end
