!Series_title	"Short Read Sequencing Data of the Transcriptome of the Rheumatoid Arthritis and Treated with Acupuncture or not"
!Series_geo_accession	"GSE58978"
!Series_status	"Public on Dec 31 2016"
!Series_submission_date	"Jul 01 2014"
!Series_last_update_date	"May 15 2019"
!Series_pubmed_id	"28008941"
!Series_summary	"We report the application of Illumina Hiseq2000 sequencing technology for high-throughput profiling of the transcriptome of the rheumatoid arthritis (RA) rat model induced by collagen type II (CIA) and treated with acupuncture or not."
!Series_overall_design	"2 rats in total, i.e., 2 replicates RA rat model before treatment (RA1, 2) for 3 in vitro culture conditions (standard serum FBS, blood serum from animals acupuncture treated AP+, blood serum from animals acupuncture untreated AP-)."
!Series_type	"Expression profiling by high throughput sequencing"
!Series_contributor	"Valentina,,Devescovi"
!Series_contributor	"Christine,,Nardini"
!Series_contributor	"Yuanhua,,Liu"
!Series_sample_id	"GSM1423259 GSM1423260 GSM1423262 GSM1423263 GSM1423265 GSM1423266 "
!Series_contact_name	"Christine,,Nardini"
!Series_contact_email	"xtalla@gmail.com"
!Series_contact_institute	"CAS-MPG Partner Institute for Computational Biology"
!Series_contact_address	"320 Yueyang Road"
!Series_contact_city	"Shanghai"
!Series_contact_zip/postal_code	"200031"
!Series_contact_country	"China"
!Series_supplementary_file	"ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE58nnn/GSE58978/suppl/GSE58978_Illumina_Hiseq2000_Acupuncture_SFB_expr.txt.gz"
!Series_platform_id	"GPL14844"
!Series_platform_taxid	"10116"
!Series_sample_taxid	"10116"
!Series_relation	"BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA254036"
!Series_relation	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP043707"

!Sample_title	"RAFLS from RA before therapy, standard condition replicate 1"	"RAFLS from RA before therapy, standard condition replicate 2"	"RAFLS from RA before therapy, untreated replicate 1"	"RAFLS from RA before therapy, untreated replicate 2"	"RAFLS from RA before therapy, AP treated replicate 1"	"RAFLS from RA before therapy, AP treated replicate 2"
!Sample_geo_accession	"GSM1423259"	"GSM1423260"	"GSM1423262"	"GSM1423263"	"GSM1423265"	"GSM1423266"
!Sample_status	"Public on Dec 31 2016"	"Public on Dec 31 2016"	"Public on Dec 31 2016"	"Public on Dec 31 2016"	"Public on Dec 31 2016"	"Public on Dec 31 2016"
!Sample_submission_date	"Jul 01 2014"	"Jul 01 2014"	"Jul 01 2014"	"Jul 01 2014"	"Jul 01 2014"	"Jul 01 2014"
!Sample_last_update_date	"May 15 2019"	"May 15 2019"	"May 15 2019"	"May 15 2019"	"May 15 2019"	"May 15 2019"
!Sample_type	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"
!Sample_channel_count	"1"	"1"	"1"	"1"	"1"	"1"
!Sample_source_name_ch1	"RA Synovial Fibroblasts Like cells"	"RA Synovial Fibroblasts Like cells"	"RA Synovial Fibroblasts Like cells"	"RA Synovial Fibroblasts Like cells"	"RA Synovial Fibroblasts Like cells"	"RA Synovial Fibroblasts Like cells"
!Sample_organism_ch1	"Rattus norvegicus"	"Rattus norvegicus"	"Rattus norvegicus"	"Rattus norvegicus"	"Rattus norvegicus"	"Rattus norvegicus"
!Sample_characteristics_ch1	"strain: Wistar rat"	"strain: Wistar rat"	"strain: Wistar rat"	"strain: Wistar rat"	"strain: Wistar rat"	"strain: Wistar rat"
!Sample_characteristics_ch1	"disease state: rheumatoid arthritis"	"disease state: rheumatoid arthritis"	"disease state: rheumatoid arthritis"	"disease state: rheumatoid arthritis"	"disease state: rheumatoid arthritis"	"disease state: rheumatoid arthritis"
!Sample_characteristics_ch1	"treatment: control"	"treatment: control"	"treatment: untreated"	"treatment: untreated"	"treatment: acupuncture"	"treatment: acupuncture"
!Sample_characteristics_ch1	"gender: female"	"gender: female"	"gender: female"	"gender: female"	"gender: female"	"gender: female"
!Sample_characteristics_ch1	"cell type: rat synovium fibroblast"	"cell type: rat synovium fibroblast"	"cell type: rat synovium fibroblast"	"cell type: rat synovium fibroblast"	"cell type: rat synovium fibroblast"	"cell type: rat synovium fibroblast"
!Sample_treatment_protocol_ch1	"RAFLS cuture were divided in three groups and mantained in different conditions, i.e. medium additioned with fetal bovine serum (FBS), blood serum from AP treated animals (AP+), blood serum from untreated animals (AP-)"	"RAFLS cuture were divided in three groups and mantained in different conditions, i.e. medium additioned with fetal bovine serum (FBS), blood serum from AP treated animals (AP+), blood serum from untreated animals (AP-)"	"RAFLS cuture were divided in three groups and mantained in different conditions, i.e. medium additioned with fetal bovine serum (FBS), blood serum from AP treated animals (AP+), blood serum from untreated animals (AP-)"	"RAFLS cuture were divided in three groups and mantained in different conditions, i.e. medium additioned with fetal bovine serum (FBS), blood serum from AP treated animals (AP+), blood serum from untreated animals (AP-)"	"RAFLS cuture were divided in three groups and mantained in different conditions, i.e. medium additioned with fetal bovine serum (FBS), blood serum from AP treated animals (AP+), blood serum from untreated animals (AP-)"	"RAFLS cuture were divided in three groups and mantained in different conditions, i.e. medium additioned with fetal bovine serum (FBS), blood serum from AP treated animals (AP+), blood serum from untreated animals (AP-)"
!Sample_growth_protocol_ch1	"RA Synovial Fibroblasts Like cells extracted from synovium tissue were grown in 75cm2 tissue colture flasks and maintained in a 37ºC, 5% CO2-95% O2 atmosphere."	"RA Synovial Fibroblasts Like cells extracted from synovium tissue were grown in 75cm2 tissue colture flasks and maintained in a 37ºC, 5% CO2-95% O2 atmosphere."	"RA Synovial Fibroblasts Like cells extracted from synovium tissue were grown in 75cm2 tissue colture flasks and maintained in a 37ºC, 5% CO2-95% O2 atmosphere."	"RA Synovial Fibroblasts Like cells extracted from synovium tissue were grown in 75cm2 tissue colture flasks and maintained in a 37ºC, 5% CO2-95% O2 atmosphere."	"RA Synovial Fibroblasts Like cells extracted from synovium tissue were grown in 75cm2 tissue colture flasks and maintained in a 37ºC, 5% CO2-95% O2 atmosphere."	"RA Synovial Fibroblasts Like cells extracted from synovium tissue were grown in 75cm2 tissue colture flasks and maintained in a 37ºC, 5% CO2-95% O2 atmosphere."
!Sample_molecule_ch1	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"
!Sample_extract_protocol_ch1	"Once  in vitro cultures ended, cells were harvested enzimatically with trypsin (Pierce) treatment, collected by centrifuged and resuspended in RNA Later before  -80ºC storage. Total RNA was then extracted and purified using Trizol Reagent (Invitrogen) according to the manufacturer's instructions, checked for the RIN number to inspect RNA integrity by the Agilent Bioanalyzer 2100 following the manufacturer's procedures of RNA 6000. Nano Kit  (Cat#5067-1511, Agilent Technologies)."	"Once  in vitro cultures ended, cells were harvested enzimatically with trypsin (Pierce) treatment, collected by centrifuged and resuspended in RNA Later before  -80ºC storage. Total RNA was then extracted and purified using Trizol Reagent (Invitrogen) according to the manufacturer's instructions, checked for the RIN number to inspect RNA integrity by the Agilent Bioanalyzer 2100 following the manufacturer's procedures of RNA 6000. Nano Kit  (Cat#5067-1511, Agilent Technologies)."	"Once  in vitro cultures ended, cells were harvested enzimatically with trypsin (Pierce) treatment, collected by centrifuged and resuspended in RNA Later before  -80ºC storage. Total RNA was then extracted and purified using Trizol Reagent (Invitrogen) according to the manufacturer's instructions, checked for the RIN number to inspect RNA integrity by the Agilent Bioanalyzer 2100 following the manufacturer's procedures of RNA 6000. Nano Kit  (Cat#5067-1511, Agilent Technologies)."	"Once  in vitro cultures ended, cells were harvested enzimatically with trypsin (Pierce) treatment, collected by centrifuged and resuspended in RNA Later before  -80ºC storage. Total RNA was then extracted and purified using Trizol Reagent (Invitrogen) according to the manufacturer's instructions, checked for the RIN number to inspect RNA integrity by the Agilent Bioanalyzer 2100 following the manufacturer's procedures of RNA 6000. Nano Kit  (Cat#5067-1511, Agilent Technologies)."	"Once  in vitro cultures ended, cells were harvested enzimatically with trypsin (Pierce) treatment, collected by centrifuged and resuspended in RNA Later before  -80ºC storage. Total RNA was then extracted and purified using Trizol Reagent (Invitrogen) according to the manufacturer's instructions, checked for the RIN number to inspect RNA integrity by the Agilent Bioanalyzer 2100 following the manufacturer's procedures of RNA 6000. Nano Kit  (Cat#5067-1511, Agilent Technologies)."	"Once  in vitro cultures ended, cells were harvested enzimatically with trypsin (Pierce) treatment, collected by centrifuged and resuspended in RNA Later before  -80ºC storage. Total RNA was then extracted and purified using Trizol Reagent (Invitrogen) according to the manufacturer's instructions, checked for the RIN number to inspect RNA integrity by the Agilent Bioanalyzer 2100 following the manufacturer's procedures of RNA 6000. Nano Kit  (Cat#5067-1511, Agilent Technologies)."
!Sample_extract_protocol_ch1	"The total RNA samples were first treated with Dnase I to degrade any possible DNA contamination. Then the mRNA was enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA was fragmented into short fragments (~200bp). Then the first strand of cDNA was sythesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second strand. The double strand cDNA was purified with magnetic beads. End reparation and 3'-end single nucleotide A addtion was then performed. Finally sequencing adaptors were ligated to the fragments. The fragments were enriched by PCR amplification. During the QC step, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR system were used to qualify and quantify the sample library."	"The total RNA samples were first treated with Dnase I to degrade any possible DNA contamination. Then the mRNA was enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA was fragmented into short fragments (~200bp). Then the first strand of cDNA was sythesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second strand. The double strand cDNA was purified with magnetic beads. End reparation and 3'-end single nucleotide A addtion was then performed. Finally sequencing adaptors were ligated to the fragments. The fragments were enriched by PCR amplification. During the QC step, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR system were used to qualify and quantify the sample library."	"The total RNA samples were first treated with Dnase I to degrade any possible DNA contamination. Then the mRNA was enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA was fragmented into short fragments (~200bp). Then the first strand of cDNA was sythesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second strand. The double strand cDNA was purified with magnetic beads. End reparation and 3'-end single nucleotide A addtion was then performed. Finally sequencing adaptors were ligated to the fragments. The fragments were enriched by PCR amplification. During the QC step, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR system were used to qualify and quantify the sample library."	"The total RNA samples were first treated with Dnase I to degrade any possible DNA contamination. Then the mRNA was enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA was fragmented into short fragments (~200bp). Then the first strand of cDNA was sythesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second strand. The double strand cDNA was purified with magnetic beads. End reparation and 3'-end single nucleotide A addtion was then performed. Finally sequencing adaptors were ligated to the fragments. The fragments were enriched by PCR amplification. During the QC step, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR system were used to qualify and quantify the sample library."	"The total RNA samples were first treated with Dnase I to degrade any possible DNA contamination. Then the mRNA was enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA was fragmented into short fragments (~200bp). Then the first strand of cDNA was sythesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second strand. The double strand cDNA was purified with magnetic beads. End reparation and 3'-end single nucleotide A addtion was then performed. Finally sequencing adaptors were ligated to the fragments. The fragments were enriched by PCR amplification. During the QC step, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR system were used to qualify and quantify the sample library."	"The total RNA samples were first treated with Dnase I to degrade any possible DNA contamination. Then the mRNA was enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA was fragmented into short fragments (~200bp). Then the first strand of cDNA was sythesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second strand. The double strand cDNA was purified with magnetic beads. End reparation and 3'-end single nucleotide A addtion was then performed. Finally sequencing adaptors were ligated to the fragments. The fragments were enriched by PCR amplification. During the QC step, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR system were used to qualify and quantify the sample library."
!Sample_taxid_ch1	"10116"	"10116"	"10116"	"10116"	"10116"	"10116"
!Sample_description	"Gene expression data from RA rats before therapy, cultivated in standard condition (FBS)"	"Gene expression data from RA rats before therapy, cultivated in standard condition (FBS)"	"Gene expression data from healthy rats before therapy"	"Gene expression data from healthy rats before therapy"	"Gene expression data from RA rats after 18 days AP treatment"	"Gene expression data from RA rats after 18 days AP treatment"
!Sample_description	"RA1_FBS"	"RA2_FBS"	"RA1_AP-"	"RA2_AP-"	"RA1_AP+"	"RA2_AP+"
!Sample_description	"FBS_1"	"FBS_2"	"APM_1"	"APM_2"	"APP_1"	"APP_2"
!Sample_data_processing	"Base calling was done with OLB (v1.9.4) and the raw sequences and quality scores (Phred64) were obtained in fastq format."	"Base calling was done with OLB (v1.9.4) and the raw sequences and quality scores (Phred64) were obtained in fastq format."	"Base calling was done with OLB (v1.9.4) and the raw sequences and quality scores (Phred64) were obtained in fastq format."	"Base calling was done with OLB (v1.9.4) and the raw sequences and quality scores (Phred64) were obtained in fastq format."	"Base calling was done with OLB (v1.9.4) and the raw sequences and quality scores (Phred64) were obtained in fastq format."	"Base calling was done with OLB (v1.9.4) and the raw sequences and quality scores (Phred64) were obtained in fastq format."
!Sample_data_processing	"Low quality reads were filtered from the raw data to obtain high quality reads. The filtered reads include: 1) reads with adapter sequences, 2) reads in which the percentage of unknown bases (N) is greater than 10%, 3) reads in which more than 50% bases whose quality value <= 5. This step was done by the company."	"Low quality reads were filtered from the raw data to obtain high quality reads. The filtered reads include: 1) reads with adapter sequences, 2) reads in which the percentage of unknown bases (N) is greater than 10%, 3) reads in which more than 50% bases whose quality value <= 5. This step was done by the company."	"Low quality reads were filtered from the raw data to obtain high quality reads. The filtered reads include: 1) reads with adapter sequences, 2) reads in which the percentage of unknown bases (N) is greater than 10%, 3) reads in which more than 50% bases whose quality value <= 5. This step was done by the company."	"Low quality reads were filtered from the raw data to obtain high quality reads. The filtered reads include: 1) reads with adapter sequences, 2) reads in which the percentage of unknown bases (N) is greater than 10%, 3) reads in which more than 50% bases whose quality value <= 5. This step was done by the company."	"Low quality reads were filtered from the raw data to obtain high quality reads. The filtered reads include: 1) reads with adapter sequences, 2) reads in which the percentage of unknown bases (N) is greater than 10%, 3) reads in which more than 50% bases whose quality value <= 5. This step was done by the company."	"Low quality reads were filtered from the raw data to obtain high quality reads. The filtered reads include: 1) reads with adapter sequences, 2) reads in which the percentage of unknown bases (N) is greater than 10%, 3) reads in which more than 50% bases whose quality value <= 5. This step was done by the company."
!Sample_data_processing	"The clean reads were aligned to rat genome UCSC rn4 (downloaded from iGENOME http://support.illumina.com/sequencing/sequencing_software/igenome.ilmn )with the aligner bowtie2 (2.1.0). Parameters: bowtie2 -N 1 -p 30 -x rn4 -U clean_data.fq.gz -S align.sam"	"The clean reads were aligned to rat genome UCSC rn4 (downloaded from iGENOME http://support.illumina.com/sequencing/sequencing_software/igenome.ilmn )with the aligner bowtie2 (2.1.0). Parameters: bowtie2 -N 1 -p 30 -x rn4 -U clean_data.fq.gz -S align.sam"	"The clean reads were aligned to rat genome UCSC rn4 (downloaded from iGENOME http://support.illumina.com/sequencing/sequencing_software/igenome.ilmn )with the aligner bowtie2 (2.1.0). Parameters: bowtie2 -N 1 -p 30 -x rn4 -U clean_data.fq.gz -S align.sam"	"The clean reads were aligned to rat genome UCSC rn4 (downloaded from iGENOME http://support.illumina.com/sequencing/sequencing_software/igenome.ilmn )with the aligner bowtie2 (2.1.0). Parameters: bowtie2 -N 1 -p 30 -x rn4 -U clean_data.fq.gz -S align.sam"	"The clean reads were aligned to rat genome UCSC rn4 (downloaded from iGENOME http://support.illumina.com/sequencing/sequencing_software/igenome.ilmn )with the aligner bowtie2 (2.1.0). Parameters: bowtie2 -N 1 -p 30 -x rn4 -U clean_data.fq.gz -S align.sam"	"The clean reads were aligned to rat genome UCSC rn4 (downloaded from iGENOME http://support.illumina.com/sequencing/sequencing_software/igenome.ilmn )with the aligner bowtie2 (2.1.0). Parameters: bowtie2 -N 1 -p 30 -x rn4 -U clean_data.fq.gz -S align.sam"
!Sample_data_processing	"The aligned reads were quantified by HTseq (0.5.4p3) using the UCSC rn4 annotation (GTF file downloaded from iGENOME). Parameters: htseq-count -m intersection-nonempty -s no -a 0 -t exon -i transcript_id"	"The aligned reads were quantified by HTseq (0.5.4p3) using the UCSC rn4 annotation (GTF file downloaded from iGENOME). Parameters: htseq-count -m intersection-nonempty -s no -a 0 -t exon -i transcript_id"	"The aligned reads were quantified by HTseq (0.5.4p3) using the UCSC rn4 annotation (GTF file downloaded from iGENOME). Parameters: htseq-count -m intersection-nonempty -s no -a 0 -t exon -i transcript_id"	"The aligned reads were quantified by HTseq (0.5.4p3) using the UCSC rn4 annotation (GTF file downloaded from iGENOME). Parameters: htseq-count -m intersection-nonempty -s no -a 0 -t exon -i transcript_id"	"The aligned reads were quantified by HTseq (0.5.4p3) using the UCSC rn4 annotation (GTF file downloaded from iGENOME). Parameters: htseq-count -m intersection-nonempty -s no -a 0 -t exon -i transcript_id"	"The aligned reads were quantified by HTseq (0.5.4p3) using the UCSC rn4 annotation (GTF file downloaded from iGENOME). Parameters: htseq-count -m intersection-nonempty -s no -a 0 -t exon -i transcript_id"
!Sample_data_processing	"The raw read counts were normalized to CPM (count per million) by the R package edgR v3.4.2."	"The raw read counts were normalized to CPM (count per million) by the R package edgR v3.4.2."	"The raw read counts were normalized to CPM (count per million) by the R package edgR v3.4.2."	"The raw read counts were normalized to CPM (count per million) by the R package edgR v3.4.2."	"The raw read counts were normalized to CPM (count per million) by the R package edgR v3.4.2."	"The raw read counts were normalized to CPM (count per million) by the R package edgR v3.4.2."
!Sample_data_processing	"Genome_build: UCSC rn4"	"Genome_build: UCSC rn4"	"Genome_build: UCSC rn4"	"Genome_build: UCSC rn4"	"Genome_build: UCSC rn4"	"Genome_build: UCSC rn4"
!Sample_data_processing	"Supplementary_files_format_and_content: The transcriptomic expression was summarized in a tab delimited matrix: the rows are REFSEQ_ID, the columns A-H are annotations for each ID, and columns I-Q are the expression (CPM) of respective sample."	"Supplementary_files_format_and_content: The transcriptomic expression was summarized in a tab delimited matrix: the rows are REFSEQ_ID, the columns A-H are annotations for each ID, and columns I-Q are the expression (CPM) of respective sample."	"Supplementary_files_format_and_content: The transcriptomic expression was summarized in a tab delimited matrix: the rows are REFSEQ_ID, the columns A-H are annotations for each ID, and columns I-Q are the expression (CPM) of respective sample."	"Supplementary_files_format_and_content: The transcriptomic expression was summarized in a tab delimited matrix: the rows are REFSEQ_ID, the columns A-H are annotations for each ID, and columns I-Q are the expression (CPM) of respective sample."	"Supplementary_files_format_and_content: The transcriptomic expression was summarized in a tab delimited matrix: the rows are REFSEQ_ID, the columns A-H are annotations for each ID, and columns I-Q are the expression (CPM) of respective sample."	"Supplementary_files_format_and_content: The transcriptomic expression was summarized in a tab delimited matrix: the rows are REFSEQ_ID, the columns A-H are annotations for each ID, and columns I-Q are the expression (CPM) of respective sample."
!Sample_platform_id	"GPL14844"	"GPL14844"	"GPL14844"	"GPL14844"	"GPL14844"	"GPL14844"
!Sample_contact_name	"Christine,,Nardini"	"Christine,,Nardini"	"Christine,,Nardini"	"Christine,,Nardini"	"Christine,,Nardini"	"Christine,,Nardini"
!Sample_contact_email	"xtalla@gmail.com"	"xtalla@gmail.com"	"xtalla@gmail.com"	"xtalla@gmail.com"	"xtalla@gmail.com"	"xtalla@gmail.com"
!Sample_contact_institute	"CAS-MPG Partner Institute for Computational Biology"	"CAS-MPG Partner Institute for Computational Biology"	"CAS-MPG Partner Institute for Computational Biology"	"CAS-MPG Partner Institute for Computational Biology"	"CAS-MPG Partner Institute for Computational Biology"	"CAS-MPG Partner Institute for Computational Biology"
!Sample_contact_address	"320 Yueyang Road"	"320 Yueyang Road"	"320 Yueyang Road"	"320 Yueyang Road"	"320 Yueyang Road"	"320 Yueyang Road"
!Sample_contact_city	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"
!Sample_contact_zip/postal_code	"200031"	"200031"	"200031"	"200031"	"200031"	"200031"
!Sample_contact_country	"China"	"China"	"China"	"China"	"China"	"China"
!Sample_data_row_count	"0"	"0"	"0"	"0"	"0"	"0"
!Sample_instrument_model	"Illumina HiSeq 2000"	"Illumina HiSeq 2000"	"Illumina HiSeq 2000"	"Illumina HiSeq 2000"	"Illumina HiSeq 2000"	"Illumina HiSeq 2000"
!Sample_library_selection	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"
!Sample_library_source	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"
!Sample_library_strategy	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"
!Sample_relation	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN02898994"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN02898995"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN02898997"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN02898998"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN02899000"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN02899001"
!Sample_relation	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX642765"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX642766"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX642768"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX642769"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX642771"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX642772"
!Sample_supplementary_file_1	"NONE"	"NONE"	"NONE"	"NONE"	"NONE"	"NONE"
!series_matrix_table_begin
"ID_REF"	"GSM1423259"	"GSM1423260"	"GSM1423262"	"GSM1423263"	"GSM1423265"	"GSM1423266"
!series_matrix_table_end
