|
DVID
|
1017587 |
|
VISID
|
TVIS10017445 |
|
Chromosome
|
chr16 |
|
GRCh38 Location
|
51319900, 51320015 |
|
Disease
|
Carcinoma, Hepatocellular |
|
Sample
|
Tumor |
|
Virus Reference Genome
|
Not given |
Literature Information
|
PubMed PMID
|
26397742
|
|
Year
|
2015 Nov;12(5):6855-60 |
|
Journal
|
Molecular medicine reports |
|
Title
|
Significance of the quantitative measurement of the chr16: 51320015 integration site in hepatocytes of patients with chronic hepatitis B. |
|
Author
|
Ruan P,Dai X,Sun Z,Zhou C,Yang F |
|
Evidence
|
The present study reported the presence of a hepatitis B virus (HBV) major integration site (MIS) chr16: 51320015 and discussed the significance of quantitative measurement of this site. A total of 30 hepatitis B e antigen (HBeAg) positive (+) and 30 HBeAg negative (-) patients with chronic hepatitis B (CHB) were enrolled in the present study, and the levels of intrahepatic (IH) covalently closed circular DNA (cccDNA), serum HBV DNA and hepatitis B surface antigen (HBsAg) were detected. Conventional reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Sanger sequencing were designed to verify the chr16: 51320015 integration site, and the copy numbers of this site were measured using molecular clone and SYBR Green I RT-qPCR. This site was found to be present in the hepatocytes of all the enrolled patients, and the average number of copies was 1.46x10-2 +/- 4.94x10-2 copies/cell (3.48x10-5-0.212 copies/cell). No significant difference in the copy numbers of this site were observed between the HBeAg (+) (1.43 +/- 9.79x10-1 copies/cell) and HBeAg (-) patients (6.58x10-2 +/- 2.47x10-2 copies/cell; P>0.05), which were positively correlated with the levels of serum HBsAg (P=0.0038), but were not correlated with the levels of IH cccDNA (P=0.7785). In conclusion, the chr16:51320015 integration site may be a novel site, which persists in a several patients with HBV infection, and may accumulate in the hepatocytes due to clonal expansion. The diagnostic and therapeutic values of this site require further investigation.
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HBV VIS Detail Information
