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Basic Characteristics of Mutations
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Mutation Site
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Y98F |
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Mutation Site Sentence
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Among the mutants examined was a virus containing a Y98F substitution at a conserved position in the receptor binding site that leads to a 20-fold reduction in binding. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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HA |
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Standardized Encoding Gene
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HA
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Influenza A
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Immune
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- |
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Target Gene
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-
|
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
|
- |
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Location
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Aichi |
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Literature Information
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PMID
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18353965
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Title
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Infectivity studies of influenza virus hemagglutinin receptor binding site mutants in mice
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Author
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Meisner J,Szretter KJ,Bradley KC,Langley WA,Li ZN,Lee BJ,Thoennes S,Martin J,Skehel JJ,Russell RJ,Katz JM,Steinhauer DA
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Journal
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Journal of virology
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Journal Info
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2008 May;82(10):5079-83
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Abstract
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The replicative properties of influenza virus hemagglutinin (HA) mutants with altered receptor binding characteristics were analyzed following intranasal inoculation of mice. Among the mutants examined was a virus containing a Y98F substitution at a conserved position in the receptor binding site that leads to a 20-fold reduction in binding. This mutant can replicate as well as wild-type (WT) virus in MDCK cells and in embryonated chicken eggs but is highly attenuated in mice, exhibiting titers in lungs more than 1,000-fold lower than those of the WT. The capacity of the Y98F mutant to induce antibody responses and the structural locations of HA reversion mutations are examined.
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Sequence Data
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-
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