| 33384922 |
The effect of acupuncture stimulation on alleviating emotional changes due to acute alcohol administration and the possibility of sigma(1) receptor involvement. |
Integr Med Res. 2021 Jun;10(2):100497. doi: 10.1016/j.imr.2020.100497. Epub 2020 Aug 12. |
2021 Jun |
BACKGROUND: Most ETOH addiction preclinical studies have focused on the rewards of chronic ETOH self-administration or the ETOH reinstatement model. Acute ETOH administration studies are scarce despite the potential of ETOH to cause sedation, intoxication and reduced acute functional tolerance. Here, we established a rat model of acute ETOH administration induced by an intraperitoneal injection of 1 g/kg ethanol and assessed the similarities in physiological and behavioral effects between acupuncture and Sigma1 R antagonists. METHODS: Male Wistar rats (300-330 g) received pretreatment with (1) saline injection, (2) saline + mechanical stimulation using a mechanical acupuncture instrument (MAI) for acupuncture at the Shenmen (HT7), (3) ETOH (1 g/kg) injection, (4) ETOH + HT7, or (5) the selective sigma(1) R antagonist BD 1047 (3, 10, or 30 mg/kg, intraperitoneal (IP) injection). ETOH (1 g/kg) or saline was IP injected after 10 min. Then, ETOH-induced immobility was evaluated in an open field arena, ultrasonic vocalizations (USVs) indicating ethanol-induced emotional changes were recorded in a recording chamber, and the rats were sacrificed for the analysis of protein levels of sigma(1) R in several regions of the brain. RESULTS: Acute ethanol exposure increased the immobile time, 22-kHz USVs, and protein levels of sigma(1) R in the ventral tegmental area (VTA). However, pretreatment with acupuncture at HT7 induced recovery of immobile time, reduced 22-kHz USVs, and regulated the protein levels of sigma1 R in the VTA. These effects have similarities with IP injection of BD 1047 (10 mg/kg). CONCLUSION: This study showed that acupuncture at HT7 regulates immobility and 22-kHz USVs via Sigma(1) R in the VTA upon acute ETOH exposure."
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