Evidence Details for Bcl2
PMID Title Journal Year Abstract
28507480 Electro-acupuncture promotes the proliferation of neural stem cells and the survival of neurons by downregulating miR-449a in rat with spinal cord injury. EXCLI J. 2017 Mar 23;16:363-374. doi: 10.17179/excli2017-123. eCollection 2017. 2017 The aim of this study is to investigate the mechanism of electro-acupuncture (EA) on the recovery of injured spinal cord. Rats were randomly divided into normal control, sham-operated, SCI, SCI+EA group and T10 segment spinal cord injury (SCI) rat model was established by the modified Allen's method. After 7 days, the mRNA and protein expression of Nestin, neuron specific nuclear protein (NeuN) and calcitonin gene related peptide (CGRP) were detected by real time RT-PCR, Western blot and immunohistochemistry respectively. The protein expression of cleaved caspase 3, Bax, Bcl-2, TNF-alpha and IL-1beta were also detected by Western blot. MicroRNA 449a (miR-449a) expression was also compared. Further, 12 SCI rats were randomly divided into EA and miR subgroups (EA + miR-449a agomir injection). The expression of Nestin, NeuN, CGRP, cleaved caspase 3, Bax, Bcl-2, TNF-alpha, IL-1beta and miR-449a was compared. The direct interaction of miR-449a and CGRP mRNA was assessed by dual luciferase reporter assay. At day 7, compared with sham-operated group, miR-449a expression in SCI group was significantly increased (P < 0.05), and NeuN and CGRP mRNA and protein expression was markedly decreased (P < 0.05), but protein levels of Nestin, cleaved caspase 3, TNF-alpha, IL-1beta and the ratio of Bax/Bcl-2 in SCI group were significantly increased (P < 0.05). The EA treatment significantly reduced miR-449a level and cleaved caspase 3, TNF-alpha, IL-1beta level and the ratio of Bax/Bcl-2 (P < 0.01), but substantially increased Nestin, NeuN and CGRP expression (P < 0.05 or 0.01). High level of miR-449a in miR subgroup was accompanied by decreased expression of Nestin, NeuN and CGRP and increased expression of cleaved caspase 3, TNF-alpha, IL-1beta and elevation of the ratio of Bax/Bcl-2 (P < 0.05), suggesting miR-449a inhibits the effects of EA on NSCs and neurons. Luciferase reporter assay showed that miR-449a bound to the 3' UTR of CGRP, and thereby regulated CGRP expression. In conclusion, EA promotes proliferation of neural stem cells and the survival of neurons by downregulation of miR-449a expression."

Evidence Sentence: The protein expression of cleaved caspase 3, Bax, Bcl-2, TNF-alpha and IL-1beta were also detected by Western blot.
Evidence Sentence: The expression of Nestin, NeuN, CGRP, cleaved caspase 3, Bax, Bcl-2, TNF-alpha, IL-1beta and miR-449a was compared.
Evidence Sentence: At day 7, compared with sham-operated group, miR-449a expression in SCI group was significantly increased (P < 0.05), and NeuN and CGRP mRNA and protein expression was markedly decreased (P < 0.05), but protein levels of Nestin, cleaved caspase 3, TNF-alpha, IL-1beta and the ratio of Bax/Bcl-2 in SCI group were significantly increased (P < 0.05).
Evidence Sentence: The EA treatment significantly reduced miR-449a level and cleaved caspase 3, TNF-alpha, IL-1beta level and the ratio of Bax/Bcl-2 (P < 0.01), but substantially increased Nestin, NeuN and CGRP expression (P < 0.05 or 0.01).
Evidence Sentence: High level of miR-449a in miR subgroup was accompanied by decreased expression of Nestin, NeuN and CGRP and increased expression of cleaved caspase 3, TNF-alpha, IL-1beta and elevation of the ratio of Bax/Bcl-2 (P < 0.05), suggesting miR-449a inhibits the effects of EA on NSCs and neurons.
Evidence Sentence: EA inhibit the SCI-induced increase of cleaved caspase 3, Bax/Bcl-2 ratio, TNF-alpha and IL-1beta in spinal cord of rats
Evidence Sentence: The protein levels of cleaved caspase 3, Bax, Bcl-2, TNF-alpha and IL-1beta in ventral horn of spinal cord of rats were detected using Western blot and the results were shown in Figure 2(Fig.
Evidence Sentence: Densitometric analysis showed that relative protein level of cleaved caspase 3, TNF-alpha and IL-1beta and the ratio of Bax to Bcl-2 were all significantly elevated in SCI group than that in sham-operated group (P < 0.05 or 0.01).
Evidence Sentence: These results indicate that EA could suppress the SCI-stimulated elevation of cleaved caspase 3, TNF-alpha and IL-1beta, and Bax/Bcl-2 ratio; EA might inhibit cell apoptosis and inflammation in ventral horn of injured spinal cord of rats.
Evidence Sentence: In addition, protein expression detection of cleaved caspase 3, Bax, Bcl-2, TNF-alpha and IL-1beta showed that the relative expression of cleaved caspase 3, TNF-alpha and IL-1beta proteins and Bax/Bcl-2 ratio in miR group were obviously higher than that in EA group (P < 0.05 or 0.01) (Figure 3I-L(Fig.
Evidence Sentence: These results suggest that high level of miR-449a could suppress the effect of EA on the expression of Nestin, NeuN, CGRP, cleaved caspase 3, Bax, Bcl-2, TNF-alpha and IL-1beta in spinal cord of rats.