Evidence Details for Calca
PMID Title Journal Year Abstract
26419631 Electro-acupuncture decreases 5-HT, CGRP and increases NPY in the brain-gut axis in two rat models of Diarrhea-predominant irritable bowel syndrome(D-IBS). BMC Complement Altern Med. 2015 Sep 29;15:340. doi: 10.1186/s12906-015-0863-5. 2015 Sep 29 BACKGROUND: To examine whether electro-acupuncture (EA) could decrease 5-hydroxytryptamine (5-HT) and calcitonin gene-related peptide (CGRP), and increase neuro-peptide Y (NPY) in the brain-gut axis (BGA) in D-IBS using rat models. METHODS: Rats were randomly exposed to unpredictable chronic stress for 3 weeks followed by 1-hour acute restraint stress (CAS) after 7 days of rest, or daily gavage of Senna decoction (6 g/kg) plus chronic restraint stress (for a duration of 2 h, starting from 1 h prior to the gavage) for 2 weeks (ISC). The content of 5-HT, CGRP and NPY in the distal colon, spinal cord, hypothalamus was examined at the end of the treatment. RESULTS: 1. The two rat models exhibited similar characteristics, e.g., increased number of fecal pellets expelled in 1 h, decreased sacchar-intake, decreased CRD, elevated 5-HT, CGRP content and decreased NPY in the distal colon, spinal cord, hypothalamus (P < 0.05 vs. that in healthy control rats). 2. A series of equations was developed based on correlation regression analysis. The analysis results demonstrated that 5-HT mediates the changes in hypothalamus, spinal cord and colon. 5-HT and CGRP in spinal cord was closely correlated with general behavior evaluation and other transmitters in BGA. CONCLUSION: 1. In comparison to 5-HT, CGRP and NPY (particularly in the spinal cord) had closer relationship with the D-IBS symptoms induced by either stress factors or Senna decotion. 2. EA treatment could restore the brain-gut axis to balanced levels."

Evidence Sentence: Electro-acupuncture decreases 5-HT, CGRP and increases NPY in the brain-gut axis in two rat models of Diarrhea-predominant irritable bowel syndrome(D-IBS)
Evidence Sentence: To examine whether electro-acupuncture (EA) could decrease 5-hydroxytryptamine (5-HT) and calcitonin gene-related peptide (CGRP), and increase neuro-peptide Y (NPY) in the brain-gut axis (BGA) in D-IBS using rat models.
Evidence Sentence: The content of 5-HT, CGRP and NPY in the distal colon, spinal cord, hypothalamus was examined at the end of the treatment.
Evidence Sentence: The two rat models exhibited similar characteristics, e.g., increased number of fecal pellets expelled in 1 h, decreased sacchar-intake, decreased CRD, elevated 5-HT, CGRP content and decreased NPY in the distal colon, spinal cord, hypothalamus (P < 0.05 vs.
Evidence Sentence: 5-HT and CGRP in spinal cord was closely correlated with general behavior evaluation and other transmitters in BGA.
Evidence Sentence: In comparison to 5-HT, CGRP and NPY (particularly in the spinal cord) had closer relationship with the D-IBS symptoms induced by either stress factors or Senna decotion.
Evidence Sentence: The CAS and ISC rats had higher 5-HT, CGRP content and lower NPY content in the colon, the spinal cord, the hypothalamus (P < 0.05 vs.
Evidence Sentence: EA decreased 5-HT and CGRP content in the distal colon, spinal cord, hypothalamus in both CAS and ISC rats (P < 0.05, vs.
Evidence Sentence: EA treatment reduced 5-HT, CGRP content and increased NPY content in both CAS and ISC rats (Fig.
Evidence Sentence: Sacchar-intake correlated negatively with the number of fecal pellets (r = -0.694) and CGRP content of the spinal cord (r = -0.668).
Evidence Sentence: NPY content of colon was proportional to CGRP content of colon and spinal cord (R = 0.652, r2 = 0.425, R2 = 0.397), and could be described using the following equation: Y = 1240.389 + 45.471X1-37.872X2, in which the NPY content of colon and X1 and X2 are CGRP content of colon and spinal cord respectively.
Evidence Sentence: T value for regression between the CGRP content of hypothalamus and the NPY content of colon was -0.872 (P = 0.387).
Evidence Sentence: With component matrix = +-0.5 as weight, CGRP of spinal cord, CRD measurement, fecal pellets, sacchar-intake amount, CGRP of hypothalamus, CGRP of distal colon, 5-HT of spinal cord, NPY of hypothalamus were significant in the f1 factors.
Evidence Sentence: F1 had the highest factor loading in CRD measurement (based on AWR Level), body weight and CGRP content of spinal cord.
Evidence Sentence: F2 was important in fecal pellet number; 5-HT content of colon, hypothalamus and spinal cord, CGRP content of hypothalamus and colon.
Evidence Sentence: F3 was important in the sacchar-intake, NPY content of colon and hypothalamus, CGRP and 5-HT content of spinal cord.