Evidence Details for Casp1
PMID Title Journal Year Abstract
30105072 Electroacupuncture Could Influence the Expression of IL-1beta and NLRP3 Inflammasome in Hippocampus of Alzheimer's Disease Animal Model. Evid Based Complement Alternat Med. 2018 Jul 12;2018:8296824. doi: 10.1155/2018/8296824. eCollection 2018. 2018 BACKGROUND: Effective therapies for Alzheimer's disease (AD) are still being explored. Electroacupuncture with traditional Chinese medicine theory may improve spatial learning and memory abilities and glucose metabolism rates in an animal model of AD. However, the mechanism of electroacupuncture in intervention of AD is still unclear. According to recent studies of AD mechanisms, the NLRP3 inflammasome regulated the expression of IL-1beta in the brain which may mediate AD related processes. Therefore, in our study, we intend to explore the possible relation between electroacupuncture and the expression of NLRP 3 inflammasome in the hippocampus of an AD animal model. METHOD: In this study, 7.5-month-old male senescence-accelerated mouse prone 8 (SAMP8) mice were used as an AD animal model, which were randomly divided into two groups: Alzheimer's disease model group (AD group) and electroacupuncture group (EA group). In the control paradigm, 7.5-month-old male SAMR1 mice were used as the normal control group (N group). DU20, DU26, and EX-HN3 were selected as the acupuncture points, and after a 15-day treatment of electroacupuncture, we used immunohistochemistry and Western blotting to examine the expression of IL-1beta and NLRP3, ASC, and Caspase-1 in the hippocampus of the AD animal model. RESULTS: Compared with N group, IL-1beta, NLRP3, ASC, and Caspase-1 positive cells in AD group were increased, and the relative expression of all above proteins significantly increased (P < 0.01). Compared with AD group, the expression of IL-1beta, NLRP3, ASC, and Caspase-1 in EA group was significantly decreased (P < 0.01). CONCLUSION: Electroacupuncture treatment could inhibit the inflammation reaction in the hippocampus of SAMP8 mice. What is more, the possible mechanism of electroacupuncture reduced the expression of IL-1beta and NLRP3 inflammasome relative protein."

Evidence Sentence: DU20, DU26, and EX-HN3 were selected as the acupuncture points, and after a 15-day treatment of electroacupuncture, we used immunohistochemistry and Western blotting to examine the expression of IL-1beta and NLRP3, ASC, and Caspase-1 in the hippocampus of the AD animal model.
Evidence Sentence: Compared with N group, IL-1beta, NLRP3, ASC, and Caspase-1 positive cells in AD group were increased, and the relative expression of all above proteins significantly increased (P < 0.01).
Evidence Sentence: Compared with AD group, the expression of IL-1beta, NLRP3, ASC, and Caspase-1 in EA group was significantly decreased (P < 0.01).
Evidence Sentence: In addition, the expression of NLRP3 and related proteins (ASC and Caspase-1) was also increased in the AD group (highlighted by red arrows).
Evidence Sentence: We could still visually distinguish that the expression of NLRP3 and related protein (ASC and Caspase-1) positive cells in the EA group was decreased compared with the AD group, with lighter staining (highlighted by blue arrows).
Evidence Sentence: Compared with N group, the relative expression of Caspase-1 protein in hippocampus of AD group was significantly higher (P < 0.01).
Evidence Sentence: However, compared with AD group, the relative expression of Caspase-1 protein in the hippocampus of the EA group was significantly decreased (P < 0.01, Figure 2(d)).