Evidence Details for Ccl11
PMID Title Journal Year Abstract
35069759 Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation. Evid Based Complement Alternat Med. 2022 Jan 13;2022:4571981. doi: 10.1155/2022/4571981. eCollection 2022. 2022 INTRODUCTION: Emerging data show that chemokine-mediated inflammation is involved in the occurrence and maintenance of pain. Recent evidence suggests that eotaxin levels rise when dysmenorrhea happens. The purpose of this study is to investigate whether eotaxin/CC chemokine receptor 3 (CCR3) axis, a key regulatory pathway for eosinophils (EOS) recruitment, is involved in acupuncture analgesia for dysmenorrhea. METHODS: After the cold congealing dysmenorrhea (CCD) rat model prepared, animals received perpendicular needling (PN) and transverse needling (TN) at SP6, respectively, for 20 min. The CCR3 agonist CCL11 was administered 30 min prior to acupuncture. Pain behavior was assessed via a writhing response. The uterine contraction test was detected by an electrophysiological method. Eotaxin, histamine (HIS), and interleukin-6 (IL-6) levels were evaluated by ELISA. The expression of CCR3 and histamine H1 receptor (H1R) was analyzed by RT-qPCR and Western blot. The expression of EOS, mast cells (MCs), eosinophil peroxidase (EPO), and eosinophil cationic protein (ECP) was assessed by hematoxylin-eosin staining (HE), Toluidine Blue staining (TB), and immunohistochemistry, respectively. RESULTS: Acupuncture prominently attenuated the menstrual pain in CCD rats, particularly TN technique. Electrophysiological recording data showed that the increased uterine contractility was ameliorated by acupuncture. In addition, TN decreased the release of eotaxin, HIS, IL-6, and the expression of CCR3 and H1R. HE, TB staining, and immunohistochemistry experiments showed that the increased expression of EOS, MCs, EPO, and ECP in uterine tissues was reversed by TN. Furthermore, we found that the effects of TN against CCD-induced menstrual pain, increased ECP expression, and HIS level were abolished by CCL11. CONCLUSION: TN alleviated menstrual pain by improving the uterine inflammatory environment via suppressing eotaxin/CCR3 axis to weak EOS-MC activation in CCD rats. The study findings support the acupuncture as a promising approach for dysmenorrhea, meanwhile, indicating the importance of performing appropriate needling technique."

Evidence Sentence: The CCR3 agonist CCL11 was administered 30 min prior to acupuncture.
Evidence Sentence: Furthermore, we found that the effects of TN against CCD-induced menstrual pain, increased ECP expression, and HIS level were abolished by CCL11.
Evidence Sentence: To further address whether pain-relief induced by TN was dependent on the activation of the eotaxin/CCR3 axis, we injected CCR3 antagonist SB328437 and agonist CCL11.
Evidence Sentence: Administration of SB328437 could mimic the effects of TN on uterine activity, and CCL11 blocked the effects of TN on uterine contractions in CCD rats (Figures 7(a)-7(d)).
Evidence Sentence: However, after the application of CCL11 in the TN group, it markedly weakened TN-induced pain relief compared with the Model + Vehicle2 + TN group as manifested by the number of contraction wave, uterine peak-to-peak value, and degree of contraction (P < 0.01, P < 0.05; Figures 7(b)-7(d)).
Evidence Sentence: Compared to the Model + vehicle2 + TN group, the ECP expression and the HIS level were significantly increased in the Model + CCL11 + TN group (P < 0.01; Figures 8(a)-8(c)).
Evidence Sentence: Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation
Evidence Sentence: Recent evidence suggests that eotaxin levels rise when dysmenorrhea happens.
Evidence Sentence: The purpose of this study is to investigate whether eotaxin/CC chemokine receptor 3 (CCR3) axis, a key regulatory pathway for eosinophils (EOS) recruitment, is involved in acupuncture analgesia for dysmenorrhea.
Evidence Sentence: Eotaxin, histamine (HIS), and interleukin-6 (IL-6) levels were evaluated by ELISA.
Evidence Sentence: In addition, TN decreased the release of eotaxin, HIS, IL-6, and the expression of CCR3 and H1R.
Evidence Sentence: TN alleviated menstrual pain by improving the uterine inflammatory environment via suppressing eotaxin/CCR3 axis to weak EOS-MC activation in CCD rats.
Evidence Sentence: TN Reduced Eotaxin Levels and Attenuated the Expression of CCR3 in the Uterus
Evidence Sentence: To determine whether TN could modulate eotaxin/CCR3 axis, we applied ELISA to detect the eotaxin level, RT-qPCR, and Western blot to analyze the expression of CCR3.
Evidence Sentence: Compared with the blank group, the eotaxin levels in uterus and plasma were significantly increased in the model group (P < 0.01; Figures 4(a) and 4(b)).
Evidence Sentence: To investigate whether the HIS expression in uterus is associated with the eotaxin/CCR3 axis, we applied ELISA to detect the HIS level, RT-qPCR, and Western blot to analyze the expression of its receptor, H1R.
Evidence Sentence: To observe the association between eotaxin/CCR3 axis and HIS, we applied HE or TB, respectively, to analyze the main inflammatory cells, EOS and MC.
Evidence Sentence: TN at SP6 can relieve pain by attenuating the expression of eotaxin/CCR3 axis and EOS-MC activation.
Evidence Sentence: To further address whether pain-relief induced by TN was dependent on the activation of the eotaxin/CCR3 axis, we injected CCR3 antagonist SB328437 and agonist CCL11.