Evidence Details for Clec4e
PMID Title Journal Year Abstract
30106055 Acupuncture through Baihui (DU20) to Qubin (GB7) mitigates neurological impairment after intracerebral hemorrhage. Neural Regen Res. 2018 Aug;13(8):1425-1432. doi: 10.4103/1673-5374.235298. 2018 Aug Inflammation plays an important role in nerve defects caused by intracerebral hemorrhage. Repairing brain damage by inhibiting the macrophage-inducible C-type lectin/spleen tyrosine kinase (Mincle/Syk) signaling pathway is a potential new target for treating cerebral hemorrhage. In this study, we aimed to determine whether acupuncture through Baihui (DU20) to Qubin (GB7) is an effective treatment for intracerebral hemorrhage through the Mincle/Syk signaling pathway. An intracerebral hemorrhage rat model was established by autologous blood infusion into the caudate nucleus. Acupuncture through Baihui to Qubin was performed for 30 minutes, once every 12 hours, for a total of three times. Piceatannol (34.62 mg/kg), a Syk inhibitor, was intraperitoneally injected as a control. Modified neurological severity score was used to assess neurological function. Brain water content was measured. Immunohistochemistry and western blot assay were used to detect immunoreactivity and protein expression levels of Mincle, Syk, and CARD9. Real-time polymerase chain reaction was used to determine interleukin-1beta mRNA levels. Hematoxylin-eosin staining was performed to observe histopathological changes. Our results showed that acupuncture through Baihui to Qubin remarkably improved neurological function and brain water content, and inhibited immunoreactivity and expression of Mincle, Syk, CARD9, and interkeukin-1beta. Moreover, this effect was similar to piceatannol. These findings suggest that acupuncture through Baihui to Qubin can improve neurological impairment after cerebral hemorrhage by inhibiting the Mincle/Syk signaling pathway."

Evidence Sentence: Repairing brain damage by inhibiting the macrophage-inducible C-type lectin/spleen tyrosine kinase (Mincle/Syk) signaling pathway is a potential new target for treating cerebral hemorrhage.
Evidence Sentence: Repairing brain damage by inhibiting the macrophage-inducible C-type lectin/spleen tyrosine kinase (Mincle/Syk) signaling pathway is a potential new target for treating cerebral hemorrhage.
Evidence Sentence: Immunohistochemistry and western blot assay were used to detect immunoreactivity and protein expression levels of Mincle, Syk, and CARD9.
Evidence Sentence: Our results showed that acupuncture through Baihui to Qubin remarkably improved neurological function and brain water content, and inhibited immunoreactivity and expression of Mincle, Syk, CARD9, and interkeukin-1beta.
Evidence Sentence: Immunopositivity of Mincle, Syk, and CARD9 in the brain as detected by immunohistochemistry
Evidence Sentence: Compared with the ICH group, Mincle immunopositivity was decreased in the acupuncture group (P < 0.05).
Evidence Sentence: However, Mincle immunopositivity was not significantly different between the ICH and piceatannol groups (P > 0.05; Figure 4).
Evidence Sentence: Protein expression of Mincle, Syk, and CARD9 in the brain as detected by western blot assay
Evidence Sentence: Compared with the ICH group, Mincle expression levels were significantly decreased in the acupuncture group (P < 0.01).
Evidence Sentence: However, Mincle expression levels were not significantly different between the ICH and piceatannol groups (P > 0.05; Figure 5.
Evidence Sentence: Effect of acupuncture on the Mincle/Syk pathway in rats with ICH
Evidence Sentence: In this study, we aimed to determine whether acupuncture through Baihui (DU20) to Qubin (GB7) is an effective treatment for intracerebral hemorrhage through the Mincle/Syk signaling pathway.
Evidence Sentence: These findings suggest that acupuncture through Baihui to Qubin can improve neurological impairment after cerebral hemorrhage by inhibiting the Mincle/Syk signaling pathway.