| Evidence Sentence: |
Real-time PCR results revealed that ephrin-B1/B3 and EphB1/B2 mRNA expression levels were significantly increased in the spinal dorsal horns of chronic constriction injury rats. |
| Evidence Sentence: |
Electroacupuncture signifi-cantly enhanced the mRNA expression of ephrin-B3 and EphB3/B6 in the dorsal horns of neuro-pathic pain rats. |
| Evidence Sentence: |
Western blot results revealed that electroacupuncture in particular, and manual acupuncture, significantly up-regulated ephrin-B3 protein levels in rat spinal dorsal horns. |
| Evidence Sentence: |
Real-time PCR analyses were used to investigate whether the mRNA expression levels of ephrin-Bs (ephrin-B1, ephrin-B2, and ephrin-B3) and EphBs (EphB1, EphB2, EphB3, EphB4, and EphB6) in the spinal dorsal horn changed under chronic constriction injury-induced neuropathic injury and after electroacupuncture and manual acupuncture treatments. |
| Evidence Sentence: |
Ephrin-B1, ephrin-B3, EphB1, and EphB2 mRNA levels in the spinal dorsal horn were significantly enhanced after chronic constriction injury-induced neuropathic injury (P < 0.05 to P < 0.01) (Figure 2). |
| Evidence Sentence: |
Specifically, the mRNA levels for EphB3, EphB6 and ephrin-B3 were dramatically increased in the spinal dorsal horn after electroacupuncture stimulation compared with the control, chronic constriction injury and manual acupuncture groups. |
| Evidence Sentence: |
Effects of electroacupuncture and manual acupuncture on the expression of ephrin-B3 protein in the spinal dorsal horn of chronic constriction injury rats |
| Evidence Sentence: |
Because ephrin-B3 was the only ephrin-B family member that was significantly up-regulated at the mRNA level in the spinal dorsal horn region after electroacupuncture treatment (Figure 2), and because electroacupuncture treatment had a better therapeutic effect in chronic constriction injury rats than manual acupuncture treatment (Figure 1), we focused on the expression of this protein after electroacupuncture and manual acupuncture treatments. |
| Evidence Sentence: |
Inconsistent with the mRNA results (Figure 2), chronic constriction injury-induced neuropathic injury did not significantly increase ephrin-B3 protein levels in the spinal dorsal horn (Figure 3). |
| Evidence Sentence: |
However, the absorbance of the bands for ephrin-B3 protein on western blots of samples from the electroacupuncture and manual acupuncture groups was significantly higher than that in western blots of samples from the control and chronic constriction injury groups (P < 0.05 or P < 0.01; Figure 3). |
| Evidence Sentence: |
Furthermore, the ephrin-B3 protein level was more significantly increased in the electroacupuncture group than in the manual acupuncture group (P < 0.05; Figure 3). |
