| Evidence Sentence: |
Behavioral responses were recorded using a video camera and c-Fos immunohistochemistry was performed thereafter. |
| Evidence Sentence: |
Treatment of EA at BL60 significantly inhibited flinching behavior and c-fos expression induced by formalin injection into the paw, compared to a control group. |
| Evidence Sentence: |
3.2. c-Fos Immunohistochemistry |
| Evidence Sentence: |
The number of c-Fos positive neurons in the dorsal horn was counted separately for superficial layers (lamina I-II) and deep layers (III-VI). |
| Evidence Sentence: |
The representative photographs of the c-Fos positive neurons of the individual groups are shown in Figure 2. |
| Evidence Sentence: |
As shown in Figure 2, the EA-For group showed a remarkable decrease in the number of c-Fos positive neurons, compared to the Formalin group. |
| Evidence Sentence: |
In order to compare the level of c-Fos immunoreactivity in the Formalin group Group 1 with pain-free animals, a normal naive animal group Group 0 was added to this study. |
| Evidence Sentence: |
The numbers of c-Fos positive neurons in lamina I-II were as follows: Group 0, 250 +- 30.68 in the group with no treatment (Normal; n = 4); Group 1, 2501 +- 255.54 in the group injected only with formalin (Formalin; n = 7); Group 2, 1163.12 +- 219.16 in the group treated with electroacupuncture before formalin injection (EA-For; n = 8); and Group 3, 1893 +- 132.91 in the group with needle insertion before formalin injection, but not treated with electrical stimulation (Sham-For; n = 10) (left in Figure 3). |
| Evidence Sentence: |
The total numbers of the c-Fos positive neurons in lamina I through VI were 518.75 +- 71.97, 4645 +- 372.30, 2148.75 +- 406.42, and 3655 +- 274.78, respectively (right in Figure 3). |
| Evidence Sentence: |
When the Normal group Group 0 was compared to each group, the Formalin group Group 1 and the Sham-For group Group 3 showed a statistically significant increase in the number of the c-Fos-expressed neurons in each lamina (I-II: F 3,17 = 23.316; III-VI: F 3,17 = 23.546; I-VI: F 3,17 = 33.101, P < 0.05; one-way ANOVA followed by Dunnett's multiple comparison). |
| Evidence Sentence: |
When the Formalin group Group 1, the EA-For group Group 2, and the Sham-For group Group 3 were compared, however, the EA-For group Group 2 showed a statistically significant decrease in the number of c-Fos positive neurons compared to the Formalin group Group 1 in each lamina (I-II: F 2,14 = 14.711; III-VI: F 2,14 = 15.647; I-VI: F 2,14 = 21.340, P < 0.05; one-way ANOVA followed by Dunnett's multiple comparison). |
| Evidence Sentence: |
These results showed that the pretreatment of EA at BL60 significantly inhibited c-Fos expression induced by formalin injection into the paw, compared to control group. |
