| Evidence Sentence: |
In the present study, the effects of acupuncture on behavioral responses and c-Fos expression were evaluated using a formalin test in male Sprague-Dawley rats in order to clarify the analgesic effects of three different acupoints. |
| Evidence Sentence: |
The acupuncture at ST36, SP9, or BL60 reduced c-Fos expression as compared with the control group. |
| Evidence Sentence: |
c-Fos immunohistochemistry |
| Evidence Sentence: |
The effects of acupuncture stimulation on c-Fos expressions are presented in Figs. |
| Evidence Sentence: |
4 shows representative photographs of the c-Fos-positive neurons in each group, demonstrating that the activation of c-Fos-positive neurons was remarkably suppressed in the ST36-For, SP9-For and BL60-For groups compared to the formalin-only group. |
| Evidence Sentence: |
The observed numbers of c-Fos-positive neurons in the laminae I-II were 403.50+-156.19, 4461+-433.75, 2323.60+-445.27, 2235.20+-254.42, 2305.60+-261.18, and 2958.20+-309.14 in the intact (normal; n=4), formalin-only (n=5), ST36-For (n=5), SP9-For (n=5), BL60-For (n=5), and NA-For (n=5) groups, respectively. |
| Evidence Sentence: |
When compared with the intact group (403.50+-156.19), all other groups showed a significant increase in the number of c-Fos-positive neurons statistically (F5,23=14.087, p<0.05, one-way ANOVA; p<0.05, Dunnett's post-hoc multiple comparison). |
| Evidence Sentence: |
However, c-Fos expression was significantly decreased in all acupuncture treatment groups compared to the formalin-only group (F4,20=7.238, p<0.05, one-way ANOVA; p<0.05, Dunnett's post-hoc multiple comparison) (Fig. |
| Evidence Sentence: |
In the case of laminae III-VI, the numbers of c-Fos-positive neurons were 222+-82.16 (normal), 2107.08+-350.02 (formalin only), 1230.80+-339.82 (ST36-For), 1123.60+-131 (SP9-For), 942.60+-66.67 (BL60-For), and 1801.60+-300.81 (NA-For). |
| Evidence Sentence: |
In the formalin-only (2107.08+-350.02), ST36-For (1230.80+-339.82) and NA-For (1801.60+-300.81) groups, c-Fos expression was significantly increased (F5,23=6.470, p<0.05) compared to the normal group (222.02+-82.16). |
| Evidence Sentence: |
When c-Fos expression was assessed between the formalin-only group and the rest of the four formalin-treated and acupuncture groups, the c-Fos expression was significantly suppressed only in the BL60-For group (F4,20=3.453, p<0.05, one-way ANOVA; p<0.05, Dunnett's post-hoc multiple comparison) (Fig. |
| Evidence Sentence: |
All groups excluding the normal group (625.50+-238.24) showed a remarkable increase of c-Fos expression statistically (F4,20=6.470, p<0.05, one-way ANOVA; p<0.05, Dunnett's post-hoc multiple comparison). |
| Evidence Sentence: |
Fos-like immunoreactivity was examined by immunohistochemistry in the rat spinal cord. |
| Evidence Sentence: |
The values from laminae I to VI in c-Fos-positive neurons were 625.50+-238.24 (normal), 6569.00+-703.25 (Formalin), 3554.40+-777.81 (ST36-For), 3358.80+-331.15 (SP9-For), 3248.20+-315.02 (BL60-For), and 4759.80+-290.66 (NA-For). |
| Evidence Sentence: |
Except for the NA-For group, c-Fos expressions in acupuncture-treated groups were significantly lower compared to the formalin only group (F4,20=7.096, p<0.05, one-way ANOVA; p<0.05, Dunnett's post-hoc multiple comparison) (Fig. |
