Evidence Details for Gfap
PMID Title Journal Year Abstract
30151295 Scolopendra Pharmacopuncture Ameliorates Behavioral Despair in Mice Stressed by Chronic Restraint. J Pharmacopuncture. 2017 Dec;20(4):257-264. doi: 10.3831/KPI.2017.20.031. Epub 2017 Sep 25. 2017 Dec INTRODUCTION: Pharmacopuncture, which combines acupuncture with herbal medicine, is one of the newly developed acupuncture techniques that has recently been put into use. The possible mechanisms of scolopendra pharmacopuncture, as well as its potential effects on depressive symptoms, were investigated in this study by using a mouse model of chronic immobilization stress (CIS). METHODS: C57BL/6 male mice were randomly assigned into three groups: mice not stressed with restraint and injected with distilled water, mice stressed with restraint and injected with distilled water, and mice stressed with restraint injected with scolopendra pharmacopuncture at a cervical site. Behavioral tests (an open field test, tail suspension test, and forced swimming test) were carried out after two weeks of CIS and injection treatments. The expression levels of glial fibrillary acidic protein (GFAP) in the hippocampus were determined by using western blot and immunohistochemistry analyses. RESULTS: Mice exposed to CIS showed decreased behavioral activity, while scolopendra pharmacopuncture treatment significantly protected against the depressive-like behaviors induced by CIS. Moreover, scolopendra pharmacopuncture treatment increased GFAP protein levels in the hippocampi of the mice stressed by chronic immobilization. CONCLUSION: Scolopendra pharmacopuncture has an ameliorating effect on depressive behavior, which is partially mediated through protection against glial loss in the hippocampus."

Evidence Sentence: The expression levels of glial fibrillary acidic protein (GFAP) in the hippocampus were determined by using western blot and immunohistochemistry analyses.
Evidence Sentence: Moreover, scolopendra pharmacopuncture treatment increased GFAP protein levels in the hippocampi of the mice stressed by chronic immobilization.
Evidence Sentence: The protein levels of GFAP in the hippocampus in the brains of mice in the two groups stressed by chronic restraint were significantly lower than they were in the brains of the control mice that had been left undisturbed (P < 0.001).
Evidence Sentence: In contrast, the protein levels of GFAP in the hippocampus in the brains of mice that had been injected with scolopendra pharmacopuncture were significantly higher than they were in the brains of the mice that had been stressed by chronic restraint and had been administered distilled water (P < 0.001).
Evidence Sentence: The immunoreactivity of GFAP in the CA3 region of the hippocampus is shown in Fig.
Evidence Sentence: Slides from the control mice displayed moderate GFAP immunostaining in the CA3 region of the hippocampus (Figs.
Evidence Sentence: Notably, slides from stressed mice treated with scolopendra pharmacopuncture also displayed a moderate level of GFAP immunostaining, resembling that seen in the slides of the control mice, in the CA3 region of the hippocampus (Figs.