Evidence Details for Hes1
PMID Title Journal Year Abstract
25878596 Acupuncture inhibits Notch1 and Hes1 protein expression in the basal ganglia of rats with cerebral hemorrhage. Neural Regen Res. 2015 Mar;10(3):457-62. doi: 10.4103/1673-5374.153696. 2015 Mar Notch pathway activation maintains neural stem cells in a proliferating state and increases nerve repair capacity. To date, studies have rarely focused on changes or damage to signal transduction pathways during cerebral hemorrhage. Here, we examined the effect of acupuncture in a rat model of cerebral hemorrhage. We examined four groups: in the control group, rats received no treatment. In the model group, cerebral hemorrhage models were established by infusing non-heparinized blood into the brain. In the acupuncture group, modeled rats had Baihui (DU20) and Qubin (GB7) acupoints treated once a day for 30 minutes. In the DAPT group, modeled rats had 0.15 mug/mL DAPT solution (10 mL) infused into the brain. Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch1 and Hes1 protein expression in rat basal ganglia. These inhibitory effects were identical to DAPT, a Notch signaling pathway inhibitor. Our results suggest that acupuncture has a neuroprotective effect on cerebral hemorrhage by inhibiting Notch-Hes signaling pathway transduction in rat basal ganglia after cerebral hemorrhage."

Evidence Sentence: Acupuncture inhibits Notch1 and Hes1 protein expression in the basal ganglia of rats with cerebral hemorrhage
Evidence Sentence: Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch1 and Hes1 protein expression in rat basal ganglia.
Evidence Sentence: Hes1 immunoreactivity in rat basal ganglia tissue
Evidence Sentence: Hes1-immunoreactive cells were mainly expressed in vascular endothelial and glial cells.
Evidence Sentence: Hes1 immunoreactivity was observed in the control group.
Evidence Sentence: At various time points, Hes1-immunoreactive cell number was significantly increased in the model group compared with the control group (P < 0.05).
Evidence Sentence: Moreover, Hes1-immunoreactive cell number was significantly lower in the acupuncture group than the model group at all time points (P < 0.05).
Evidence Sentence: Hes1 immunoreactivity was suppressed in the DAPT group, with significant differences between DAPT and model groups at 3, 7, and 14 days (P < 0.05).
Evidence Sentence: Hes1 immunoreactivity was inhibited in both the acupuncture and DAPT groups, but no significant difference was detected at any time point (P > 0.05; Table 3, Figure 3).
Evidence Sentence: Our results suggest that acupuncture has a neuroprotective effect on cerebral hemorrhage by inhibiting Notch-Hes signaling pathway transduction in rat basal ganglia after cerebral hemorrhage.