| Evidence Sentence: |
It decreased 5-hydroxytryptamine (5-HT) levels and immunoreactive expressions in the dorsal raphe nucleus (DRN). |
| Evidence Sentence: |
It decreased 5-hydroxytryptamine (5-HT) levels and immunoreactive expressions in the dorsal raphe nucleus (DRN). |
| Evidence Sentence: |
100-Hz EA alleviated the pain-depression dyad and upregulated 5-HT in the DRN of reserpine-injected rats. |
| Evidence Sentence: |
Intracerebroventricular injection of para-chlorophenylalanine, an inhibitor of 5-HT resynthesis, suppressed the upregulation of 5-HT in the DRN by 100-Hz EA and partially counteracted the analgesic and anti-depressive effects of 100-Hz EA. |
| Evidence Sentence: |
The present study was the first to demonstrate that 5-HT in the DRN is involved in mediating the analgesic and anti-depressive effects of 100-Hz EA on the pain-depression dyad. |
| Evidence Sentence: |
The data were analyzed using a two-tailed student's t-test for the behavior results, 5-HT levels and 5-HT cell expression levels. P<0.05 was considered to indicate a statistically significant difference. |
| Evidence Sentence: |
HPLC-ECD was adopted to determine the effect of 100-Hz EA on 5-HT levels in the DRN in rats with pain-depression dyad. |
| Evidence Sentence: |
Repeated injection of reserpine significantly decreased the 5-HT levels of DRN in rats (Fig. 5; P<0.05). |
| Evidence Sentence: |
100-Hz EA significantly increased the 5-HT levels of DRN in reserpine-injected rats (P<0.05, vs. the model group). |
| Evidence Sentence: |
The upregulation of 5-HT levels in DRN by 100-Hz EA was completely abrogated by injection of pCPA (i.c.v.) in the pCPA + EA group (P<0.05, when compared to the EA group). |
| Evidence Sentence: |
Distribution of 5-HT-immunoreactive cells in the DRN of rats was investigated at bregma -7.5, -8.0 and -8.5 mm (Fig. 6A-C, respectively). |
| Evidence Sentence: |
The abundance of 5-HT-immunoreactive cells in the DRN was higher at bregma -8.00 mm than at bregma -7.50 and -8.50 mm. |
