Evidence Details for Il10
PMID Title Journal Year Abstract
21799673 Involvement of Interleukin-10 in the Anti-Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis. Evid Based Complement Alternat Med. 2011;2011:217946. doi: 10.1093/ecam/neq036. Epub 2011 Jun 5. 2011 In this study, we determined the anti-inflammatory effect of manual acupuncture at the Sanyinjiao or Spleen 6 (SP6) point on carrageenan-induced peritonitis in mice and investigated mechanisms that may underlie this effect. In the first set of experiments, male Swiss mice were allocated into five groups: the control (sterile saline), dexamethasone (DEXA), invasive sham-acupuncture (non-acupoint), SP6 acupuncture and carrageenan-treated groups. Ten minutes after needle retention or 30 min after DEXA treatment, mice received an intraperitoneal injection of carrageenan (750 mug/mouse). After 4 h, total leukocyte and differential cell counts (neutrophils and mononuclear), myeloperoxidase (MPO) activity, vascular permeability and cytokine levels were evaluated. In another set of experiments, adrenalectomized (ADX) mice were used to study the involvement of the adrenal gland on the therapeutic effects of acupuncture. Mice were allocated into two groups: the ADX and sham-operated animals (Sham ADX) that were subdivided into four subgroups each: the control (sterile saline), DEXA, SP6 acupuncture and carrageenan-treated groups. The SP6 and DEXA treatments inhibited the inflammatory cell infiltration, vascular permeability and MPO activity in carrageenan-injected mice. In addition, the SP6 treatment also increased interleukin (IL)-10 levels. In contrast, when the animals were adrenalectomized, the SP6 treatment failed to reduce total leukocyte and the plasma extravasation. In conclusion, this study clearly demonstrates the anti-inflammatory effect of SP6 acupuncture in a model of carrageenan-induced peritonitis. Our results demonstrated that SP6 acupuncture depends of the adrenal glands and increased IL-10 levels to produce its anti-inflammatory action."

Evidence Sentence: Our results demonstrated that SP6 acupuncture depends of the adrenal glands and increased IL-10 levels to produce its anti-inflammatory action.
Evidence Sentence: SP6 Acupuncture and TNF-alpha, IL-1beta and IL-10 Levels in Mice with Carrageenan-Induced Peritonitis
Evidence Sentence: Figures 4(a)-4(c) shows that TNF-alpha, IL-1beta and IL-10 could be detected in the peritoneal fluid from control groups.
Evidence Sentence: However, 4 h after carrageenan injection, TNF-alpha and IL-1beta levels increased to 137 and 3480 pg mL-1, and IL-10 levels decreased to 69 pg mL-1, relative to the control groups (62, 192 and 135 pg mL-1, resp.
Evidence Sentence: Furthermore, treatment of the animals with DEXA caused significant inhibition of the TNF-alpha and IL-1beta levels in peritoneal fluid by 86 and 1489 pg mL-1 (Figures 4(a) and 4(b)), respectively, while IL-10 levels were unchanged (Figure 4(c)).
Evidence Sentence: In sharp contrast, SP6 acupuncture did not reduce TNF-alpha and IL-1beta levels in peritoneal fluid (Figures 4(a) and 4(b)), but significantly increased the anti-inflammatory cytokine IL-10 levels (by 143 pg mL-1) after carrageenan injection (Figure 4(c)).
Evidence Sentence: In addition, the SP6 treatment also increased interleukin (IL)-10 levels.
Evidence Sentence: Involvement of Interleukin-10 in the Anti-Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis