Evidence Details for Il1b
PMID Title Journal Year Abstract
34564665 Bee Venom Alleviated Edema and Pain in Monosodium Urate Crystals-Induced Gouty Arthritis in Rat by Inhibiting Inflammation. Toxins (Basel). 2021 Sep 16;13(9):661. doi: 10.3390/toxins13090661. 2021 Sep 16 Bee venom (BV) acupuncture has anti-inflammatory and analgesic effects; therefore, it was used as a traditional Korean medicine for various musculoskeletal disorders, especially arthritis. In this study, we investigated the effect of BV on monosodium urate (MSU) crystal-induced acute gouty rats. An intra-articular injection of MSU crystal suspension (1.25 mg/site) was administered to the tibiotarsal joint of the hind paw of Sprague Dawley rats to induce MSU crystal-induced gouty arthritis. Colchicine (30 mg/kg) was orally administered 1 h before MSU crystal injection as a positive control, and BV (0.5 mg/kg) was injected into the tibiotarsal joint immediately after MSU crystal injection. The ankle thickness, mechanical allodynia, and expression of proinflammatory cytokines (TNF-alpha, IL-1beta, IL6, COX2 and iNOS) and chemokines (MIP-1alpha, MIP-1beta, MCP-1, GRO-alpha, MIP-2alpha) were then evaluated. BV reduced the expression of proinflammatory cytokines and chemokines, which are important mediators of MSU crystal-induced inflammatory responses. This anti-inflammatory effect was also confirmed histologically to attenuate synovitis and neutrophil infiltration. We demonstrated that BV markedly ameliorated ankle edema and mechanical allodynia in gouty rats. These results suggest that BV acupuncture is a potential clinical therapy for acute gouty management."

Evidence Sentence: The ankle thickness, mechanical allodynia, and expression of proinflammatory cytokines (TNF-alpha, IL-1beta, IL6, COX2 and iNOS) and chemokines (MIP-1alpha, MIP-1beta, MCP-1, GRO-alpha, MIP-2alpha) were then evaluated.
Evidence Sentence: Therefore, we examined the effect of BV on blood cell infiltration and expression of inflammatory mediators by reverse transcriptase-polymerase chain reaction (PCR), western blotting, and immunohistochemistry at 24 h after MSU injection, and found that the messenger RNAs levels of TNF-alpha, IL-1beta, IL6, COX-2, and iNOS (at 24 h) were significantly upregulated after MSU injection, but BV and Col significantly reduced their levels as compared with that of MSU injected rats (n = 3) (Figure 4A,B).
Evidence Sentence: Blood cell infiltration following MSU injection initiates inflammatory responses by producing inflammatory mediators such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, COX-2, and inducible nitric oxide synthase (iNOS).