Evidence Details for Mbp
PMID Title Journal Year Abstract
30505267 Electroacupuncture Restores Locomotor Functions After Mouse Spinal Cord Injury in Correlation With Reduction of PTEN and p53 Expression. Front Mol Neurosci. 2018 Nov 16;11:411. doi: 10.3389/fnmol.2018.00411. eCollection 2018. 2018 Background: We previously showed that electroacupuncture (EA) at Jiaji points promotes expression of adhesion molecule L1 in spinal cord tissue after mouse spinal cord injury (SCI) and contributes to recovery of neural functions. Objective: We investigated the effects of EA on downstream signaling molecules of L1 and molecules relevant to apoptosis with the aim to understand the underlying molecular mechanisms. Methods: Female C57BL/6 mice were divided into a sham group, injury group, injury+acupuncture (AP) group and injury+EA group. We investigated the changes in cognate L1-triggered signaling molecules after SCI by immunofluorescence staining and immunoblot analysis. Results: Protein levels of phosphatase and tensin homolog (PTEN) and p53 were decreased by EA at different time points after injury, whereas the levels of phosphorylated mammalian target of rapamycin (pmTOR), p-Akt and phosphorylated extracellular signal-regulatedkinase (p-Erk) were increased. Also, levels of myelin basic protein (MBP) were increased by EA. AP alone showed less pronounced changes in expression of the investigated molecules, when compared to EA. Conclusion: We propose that EA contributes to neuroprotection by inhibiting PTEN and p53 expression and by increasing the levels of pmTOR/Akt/Erk and of MBP after SCI. These observations allow novel insights into the beneficial effects of EA via L1-triggered signaling molecules after injury."

Evidence Sentence: Also, levels of myelin basic protein (MBP) were increased by EA.
Evidence Sentence: Conclusion: We propose that EA contributes to neuroprotection by inhibiting PTEN and p53 expression and by increasing the levels of pmTOR/Akt/Erk and of MBP after SCI.
Evidence Sentence: AP and EA Increase Expression of MBP After SCI
Evidence Sentence: To evaluate whether AP and EA affect expression of MBP after SCI, we performed immunofluorescence staining and immunoblot analysis.
Evidence Sentence: Figures 6A1-G3 are representative images of MBP and DAPI staining at 3 and 14 days after SCI.
Evidence Sentence: Figures 6A1-A3 represent expression of MBP in the sham group, showing a normal structure.
Evidence Sentence: At 3 days, expression of MBP was decreased in the injury group compared with the other groups.
Evidence Sentence: Compared with the injury group, levels of MBP were increased in the injury+AP group and the injury+EA group at 3 days after SCI (means +- SEM are 1.6478 +- 0.0087 and 0.5028 +- 0.011, t = 12.1294, p = 0.0001; means +- SEM are 1.9909 +- 1.4873 and 0.5028 +- 0.011, t = 12.1179, p = 0.0001, respectively).
Evidence Sentence: At 7 days after SCI, expression of MBP was enhanced in the injury+AP group and the injury+EA group when compared to the injury group (means +- SEM are 2.1689 +- 1.4873 and 3.3578 +- 0.9745, t = 2.8305, p = 0.0006; means +- SEM are 2.1689 +- 1.4873 and 3.3578 +- 0.9745, t = 2.8305, p = 0.0001, respectively).
Evidence Sentence: At all time points studied, levels of MBP were higher in the injury+EA group than in the injury group (means +- SEM are 1.5672 +- 0.0288 and 0.6724 +- 0.0054, t = 8.3945, p = 0.0001; means +- SEM are 1.881 +- 0.0227 and 1.3791 +- 0.033, t = 3.68847, p = 0.01; Figures 6H,I).
Evidence Sentence: Our observations indicate that EA promotes expression of MBP at 3, 7 and 14 days, while AP improves MBP expression only at 3 and 7 days after SCI, showing a less sustainable effect.