Evidence Details for Mtor
PMID Title Journal Year Abstract
30089868 Acupuncture modulates stress response by the mTOR signaling pathway in a rat post-traumatic stress disorder model. Sci Rep. 2018 Aug 8;8(1):11864. doi: 10.1038/s41598-018-30337-5. 2018 Aug 8 Post-traumatic stress disorder (PTSD) is a psychiatric disease that can form following exposure to a traumatic event. Acupuncture has been proposed as a beneficial treatment for PTSD, but the underlying mechanisms remain unclear. The present study investigated whether acupuncture improves depression- and anxiety-like behaviors induced using a single prolonged stress (SPS) as a PTSD rat model. In addition, we investigated whether the effects were mediated by increased mTOR activity and its downstream signaling components, which contribute to protein synthesis required for synaptic plasticity in the hippocampus. We found that acupuncture at HT8 significantly alleviated both depression- and anxiety-like behaviors induced by SPS in rats, as assessed by the forced swimming, elevated plus maze, and open field tests; this alleviation was blocked by rapamycin. The effects of acupuncture were equivalent to those exerted by fluoxetine. Acupuncture regulated protein translation in the mTOR signaling pathway and enhanced the activation of synaptic proteins, PSD95, Syn1, and GluR1 in the hippocampus. These results suggest that acupuncture exerts antidepressant and anxiolytic effects on PTSD-related symptoms by increasing protein synthesis required for synaptic plasticity via the mTOR pathway in the hippocampus. Acupuncture may be a promising treatment for patients with PTSD and play a role as an alternative PTSD treatment."

Evidence Sentence: In addition, we investigated whether the effects were mediated by increased mTOR activity and its downstream signaling components, which contribute to protein synthesis required for synaptic plasticity in the hippocampus.
Evidence Sentence: The proteins assessed were p-extracellular signal-regulated kinase (ERK) forms 1 and 2, p-Akt, p-mTOR, p-p70S6K, p-4E-BP-1, and p-CREB.
Evidence Sentence: One-way ANOVA revealed significant differences in protein expressions of p-ERK/ERK [F4,10 = 10.95, p = 0.0011], p-Akt/Akt [F4,10 = 43.05, p < 0.0001], p-mTOR/mTOR [F4,10 = 24.43, p < 0.0001], p-p70S6K/beta-actin [F4,10 = 37.26, p < 0.0001], p-4E-BP-1/beta-actin [F4,10 = 15.74, p = 0.0003], and p-CREB/beta-actin [F4,10 = 164.8, p < 0.0001] in the hippocampus.
Evidence Sentence: Post hoc analysis showed that the levels of, p-ERK (p < 0.05, Fig. 4A), p-Akt (p < 0.01, Fig. 4B), p-mTOR (p < 0.001, Fig. 4C), p-p70S6K (p < 0.01, Fig. 4D), p-4E-BP-1 (p < 0.01, Fig. 4E), and p-CREB (p < 0.001, Fig. 4F) in the SPS group were significantly decreased compared with those in the Nor group.
Evidence Sentence: Compared with values in the SPS group, the expressions of p-ERK (Fig. 4A), p-Akt (p < 0.001, Fig. 4B), p-mTOR (p < 0.001, Fig. 4C), p-p70S6K (p < 0.01, Fig. 4D), p-4E-BP-1 (p < 0.01, Fig. 4E), and p-CREB (p < 0.001, Fig. 4F) were increased by acupuncture stimulation at HT8.
Evidence Sentence: In addition, the levels of p-ERK (p < 0.01, Fig. 4A), p-Akt (p < 0.001, Fig. 4B), p-mTOR (p < 0.001, Fig. 4C), p-p70S6K (p < 0.01, Fig. 4D), p-4E-BP-1 (p < 0.01, Fig. 4E), and p-CREB (p < 0.001, Fig. 4F) were significantly increased in the SPS + Flu group compared with the SPS group.
Evidence Sentence: Pretreatment with the mTOR inhibitor rapamycin blocked the effect of HT8
Evidence Sentence: Taken together, these findings suggest that pretreatment with the mTOR inhibitor rapamycin significantly blocked the effect of HT8 on the depression- and anxiety-like behaviors.
Evidence Sentence: Therefore, the effect of HT8 treatment on EPM test was significantly blocked by pretreatment with the mTOR inhibitor, rapamycin.
Evidence Sentence: Effects of acupuncture at HT8 on the expressions of p-mTOR in the CA1, CA3 and dentate gyrus (DG) of the hippocampus using immunofluorescent analysis
Evidence Sentence: We tried to investigate which regions of hippocampus were associated with the changes of p-mTOR by acupuncture, and one-way ANOVA revealed that the densities of p-mTOR were significantly different in the CA1 [F2,17 = 15.30, p < 0.0001] and DG [F2,17 = 13.20, p = 0.0002] between the all groups (Fig. 6A,B), while there were no such difference in CA2 [F2,17 = 2.063, p = 0.1411] and CA3 [F2,17 = 3.087, p = 0.0509].
Evidence Sentence: Post hoc test showed that the level of p-mTOR in SPS group was lower than that in Nor group (p < 0.001) in both CA1 and DG.
Evidence Sentence: These results suggest that acupuncture exerts antidepressant and anxiolytic effects on PTSD-related symptoms by increasing protein synthesis required for synaptic plasticity via the mTOR pathway in the hippocampus.
Evidence Sentence: To confirm the involvement of the mTOR signaling pathway in the effect of acupuncture at HT8, we injected the rats with rapamycin (Rapa, 110 ug/ul; intranasal [i.n.]), an inhibitor of the mTOR pathway, and then performed the behavioral tests.
Evidence Sentence: Acupuncture modulates stress response by the mTOR signaling pathway in a rat post-traumatic stress disorder model
Evidence Sentence: Acupuncture regulated protein translation in the mTOR signaling pathway and enhanced the activation of synaptic proteins, PSD95, Syn1, and GluR1 in the hippocampus.
Evidence Sentence: Effects of acupuncture at HT8 on the mTOR signaling pathway in the hippocampus following SPS
Evidence Sentence: To investigate whether the antidepressant and anxiolytic effects of acupuncture are associated with the mTOR signaling pathway, we analyzed the protein expression levels of key proteins in this pathway.
Evidence Sentence: The mTOR signaling pathway is important in regulating protein translation.