| Evidence Sentence: |
To further explore its molecular mechanism, we detected protein levels of p-mTOR, mTOR, p-UlK1Ser757, and ULK1 with Western blotting. |
| Evidence Sentence: |
As illustrated in Figure 5F and Figure 5G, acupuncture enhanced the p-mTOR /mTOR ratio and p-ULK1Ser757 /ULK1 ratio on Day 7 after TBI. |
| Evidence Sentence: |
Moreover, the CO-IP results (Figure 5H) showed that acupuncture treatment accelerated the connection between mTOR and ULK1, while the connection was inhibited under rapamycin combination therapy. |
| Evidence Sentence: |
It is obvious that the activation of acupuncture on mTOR could be receded when in combination with rapamycin. |
| Evidence Sentence: |
Acupuncture promotes nerve repair through the benign regulation of mTOR-mediated neuronal autophagy in traumatic brain injury rats Acupuncture promotes nerve repair through the benign regulation of mTOR-mediated neuronal autophagy in TBI rats |
| Evidence Sentence: |
Western blotting was used to determine the protein expressions of LC3, p62, beclin1, mTOR, ULK1, p-mTOR, and p-ULK1. |
| Evidence Sentence: |
Co-immunoprecipitation (CO-IP) method was used to identify the protein interaction of mTOR and ULK1. |
| Evidence Sentence: |
In the injured cortex, the expression levels of p-mTOR/mTOR (Figure 3A and Figure 3B) and p-ULK1Ser757 /ULK1 (Figure 3A,C) in TBI rats increased, but decreased significantly after acupuncture treatment on Day 3 after TBI. |
| Evidence Sentence: |
Acupuncture accelerated the expression of p-mTOR /mTOR and p-ULK1ser757/ULK1 on Day 7 and Day 14 in TBI rats. |
| Evidence Sentence: |
Of note, 3-MA abrogated the acupuncture-mediated decrease in the p-mTOR/mTOR ratio and p-ULK1Ser757/ULK1 ratio. |
| Evidence Sentence: |
CO-IP results (Figure 4G) showed that 3-MA also enhanced the acupuncture-mediated weakening of the mTOR and ULK1 connection. |
| Evidence Sentence: |
The mTOR inhibitor rapamycin can counteract the therapeutic effect of acupuncture on TBI rats by activating autophagy |
| Evidence Sentence: |
To clarify the relationship between acupuncture and mTOR - dependent neuronal autophagy, we treated TBI rats with acupuncture combined with rapamycin (mTOR inhibitor). |
| Evidence Sentence: |
Acupuncture may regulate neuronal autophagy by modulating mTOR dependent signaling pathway |
| Evidence Sentence: |
Therefore, we inferred that the benign regulation of acupuncture on autophagy in TBI rats is mediated by the mTOR signaling pathway. |
| Evidence Sentence: |
Acupuncture has a benign regulatory effect on neuronal autophagy in different stages of TBI, possibly through the mTOR/ULK1 pathway. |
| Evidence Sentence: |
Collectively, acupuncture may mediate the autophagy of cerebral cortex neurons through the mTOR/ULK1 pathway, thus exhibiting a neuroprotective effect. |
| Evidence Sentence: |
The results above suggested that the benign protective effect of acupuncture after TBI may be closely related to mTOR-dependent signaling pathway-mediated autophagy. |
