Evidence Details for Naip6
PMID Title Journal Year Abstract
27073385 Manual acupuncture at the SJ5 (Waiguan) acupoint shows neuroprotective effects by regulating expression of the anti-apoptotic gene Bcl-2. Neural Regen Res. 2016 Feb;11(2):305-11. doi: 10.4103/1673-5374.177740. 2016 Feb Acupuncture at the SJ5 (Waiguan) acupoint has neuroprotective effects in cerebral infarction, but the underlying mechanism remains poorly understood. Here, we analyzed gene expression in healthy rat cerebellum using a pathway-focused DNA microarray to screen 113 genes associated with 18 signal transduction pathways. After 20 minutes of acupuncture at SJ5, the expression of Bcl-2 and Birc1b mRNA was markedly increased. This was confirmed by real-time reverse transcription PCR. Furthermore, western blot analysis showed that Bcl-2 protein expression remained high in the cerebellum until at least 2 hours after cessation of acupuncture. These findings indicate that acupuncture at SJ5 exerts neuroprotective effects by regulating the expression of anti-apoptotic gene Bcl-2."

Evidence Sentence: After 20 minutes of acupuncture at SJ5, the expression of Bcl-2 and Birc1b mRNA was markedly increased.
Evidence Sentence: Expression of Bcl-2 and Birc1b was elevated in distinct survival signaling pathways in the SJ5 acupuncture group compared with the control group (Figure 2; Table 2).
Evidence Sentence: Acupuncture stimulation at SJ5 increased mRNA expression of Bcl-2 and Birc1b in the cerebellum 2 hours after cessation
Evidence Sentence: To validate the gene expression data obtained from the microarray, we analyzed Bcl-2 and Birc1b mRNA expression using real-time PCR.
Evidence Sentence: Normalized mRNA levels of Bcl-2 and Birc1b in the SJ5 and non-acupoint groups were significantly higher than those in the control group (P < 0.01; Figure 3A, B).
Evidence Sentence: At 20 minutes, Bcl-2 and Birc1b expression was greater in the SJ5 and non-acupoint groups than in the control group (P < 0.001), confirming the microarray data.
Evidence Sentence: After 2 hours, Bcl-2 expression remained elevated (P < 0.01), but Birc1b expression had decreased.
Evidence Sentence: The relative expression of Bcl-2 and Birc1b mRNA, normalized against beta-actin, are shown in Figure 4.