| Evidence Sentence: |
Therefore, in our study, we intend to explore the possible relation between electroacupuncture and the expression of NLRP 3 inflammasome in the hippocampus of an AD animal model. |
| Evidence Sentence: |
Electroacupuncture Could Influence the Expression of IL-1beta and NLRP3 Inflammasome in Hippocampus of Alzheimer's Disease Animal Model |
| Evidence Sentence: |
According to recent studies of AD mechanisms, the NLRP3 inflammasome regulated the expression of IL-1beta in the brain which may mediate AD related processes. |
| Evidence Sentence: |
DU20, DU26, and EX-HN3 were selected as the acupuncture points, and after a 15-day treatment of electroacupuncture, we used immunohistochemistry and Western blotting to examine the expression of IL-1beta and NLRP3, ASC, and Caspase-1 in the hippocampus of the AD animal model. |
| Evidence Sentence: |
Compared with N group, IL-1beta, NLRP3, ASC, and Caspase-1 positive cells in AD group were increased, and the relative expression of all above proteins significantly increased (P < 0.01). |
| Evidence Sentence: |
Compared with AD group, the expression of IL-1beta, NLRP3, ASC, and Caspase-1 in EA group was significantly decreased (P < 0.01). |
| Evidence Sentence: |
What is more, the possible mechanism of electroacupuncture reduced the expression of IL-1beta and NLRP3 inflammasome relative protein. |
| Evidence Sentence: |
Expression of IL-1beta and NLRP3 Related Proteins in Hippocampus: Seeing from Immunohistochemistry |
| Evidence Sentence: |
In addition, the expression of NLRP3 and related proteins (ASC and Caspase-1) was also increased in the AD group (highlighted by red arrows). |
| Evidence Sentence: |
We could still visually distinguish that the expression of NLRP3 and related protein (ASC and Caspase-1) positive cells in the EA group was decreased compared with the AD group, with lighter staining (highlighted by blue arrows). |
| Evidence Sentence: |
Expression of IL-1beta and NLRP3 Related Proteins in Hippocampus from Western Blots |
| Evidence Sentence: |
In the Western blot results (Figure 2), the relative expression of IL-1beta and NLRP3 related proteins were obtained by comparing the gray value of each sample with beta-actin, which was set as the internal reference. |
| Evidence Sentence: |
Compared with N group, the relative expression of NLRP3 protein in the hippocampus of other two groups was significantly higher (AD group: P < 0.01; EA group: P < 0.05). |
| Evidence Sentence: |
Compared with AD group, the expression of NLRP3 protein in the hippocampus of the EA group was significantly decreased (P < 0.01, Figure 2(b)). |
