Evidence Details for Notch1
PMID Title Journal Year Abstract
25878596 Acupuncture inhibits Notch1 and Hes1 protein expression in the basal ganglia of rats with cerebral hemorrhage. Neural Regen Res. 2015 Mar;10(3):457-62. doi: 10.4103/1673-5374.153696. 2015 Mar Notch pathway activation maintains neural stem cells in a proliferating state and increases nerve repair capacity. To date, studies have rarely focused on changes or damage to signal transduction pathways during cerebral hemorrhage. Here, we examined the effect of acupuncture in a rat model of cerebral hemorrhage. We examined four groups: in the control group, rats received no treatment. In the model group, cerebral hemorrhage models were established by infusing non-heparinized blood into the brain. In the acupuncture group, modeled rats had Baihui (DU20) and Qubin (GB7) acupoints treated once a day for 30 minutes. In the DAPT group, modeled rats had 0.15 mug/mL DAPT solution (10 mL) infused into the brain. Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch1 and Hes1 protein expression in rat basal ganglia. These inhibitory effects were identical to DAPT, a Notch signaling pathway inhibitor. Our results suggest that acupuncture has a neuroprotective effect on cerebral hemorrhage by inhibiting Notch-Hes signaling pathway transduction in rat basal ganglia after cerebral hemorrhage."

Evidence Sentence: Notch pathway activation maintains neural stem cells in a proliferating state and increases nerve repair capacity.
Evidence Sentence: These inhibitory effects were identical to DAPT, a Notch signaling pathway inhibitor.
Evidence Sentence: Acupuncture inhibits Notch1 and Hes1 protein expression in the basal ganglia of rats with cerebral hemorrhage
Evidence Sentence: Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch1 and Hes1 protein expression in rat basal ganglia.
Evidence Sentence: Notch1 protein expression in rat basal ganglia tissue
Evidence Sentence: In each group, Notch1 protein expression was visible in the basal ganglia at 1, 3, 7, and 14 days after modeling.
Evidence Sentence: Compared with the control group, Notch1 protein expression was significantly higher in the model group at 1, 3, and 7 days (P < 0.01).
Evidence Sentence: Furthermore, compared with the model group, Notch1 protein expression was significantly lower in the DAPT group at each time point, particularly at 3 days (P < 0.01) although significant differences were still detected at 7 and 14 days (P < 0.05).
Evidence Sentence: At all time points, Notch1 protein expression was significantly lower in the acupuncture group than in the model group (P < 0.01), and was inhibited in the DAPT and acupuncture groups (P > 0.05; Table 1, Figure 1).
Evidence Sentence: Notch1 immunoreactivity in rat basal ganglia tissue
Evidence Sentence: Notch1-immunoreactive cells were widely distributed in the cytoplasm and nucleus.
Evidence Sentence: Weak Notch1 immunoreactivity was observed in the control group.
Evidence Sentence: Notch1-immunoreactive cell number was significantly higher in the model group than in the control group at various time points (P < 0.05), and significantly lower in the DAPT group than the model group at 1, 3 (P < 0.01), and 7 (P < 0.05) days.
Evidence Sentence: Notch1-immunoreactive cell number was lower in the acupuncture group than in the model group at 1, 3, and 7 days (P < 0.05).
Evidence Sentence: Our results suggest that acupuncture has a neuroprotective effect on cerebral hemorrhage by inhibiting Notch-Hes signaling pathway transduction in rat basal ganglia after cerebral hemorrhage.