Evidence Details for Pax8
PMID Title Journal Year Abstract
28672928 Mechanism of Mongolian medical warm acupuncture in treating insomnia by regulating miR-101a in rats with insomnia. Exp Ther Med. 2017 Jul;14(1):289-297. doi: 10.3892/etm.2017.4452. Epub 2017 May 11. 2017 Jul MicroRNAs (miRNAs or miRs) and the target genes before and after warm acupuncture at the genetic level were assessed, and the cytokines and neurotransmitters related to insomnia were studied. Male Sprague-Dawley rats were used to create PCPA insomnia rat models and randomly divided into the normal, model, warm acupuncture, and drug groups. The Dinghui Acupoint, Heyi Acupoint, and Xin Acupoint were inserted in the Mongolian medicine warm acupuncture group. The differential expression profile of microRNA in the brain tissue of the insomnia rats was determined before and after Mongolian medicine warm acupuncture for establishment of miR-101a mimics and inhibitor. qPCR was used to detect the expression level of miR-101a. Western blotting was used to detect the expression level of PAX8. The rats receiving Mongolian medicine warm acupuncture had 141 miRNAs with differential expression compared with the normal rats. The expression level of miR-101a in the cells of the hippocampus of the insomnia rats transfected with miR-101a mimics increased significantly at 72 h (P<0.05). The activity of the neuronal cells transfected with miR-101a inhibitor increased significantly at 72 h (P<0.05). The western blotting result indicated that the expression of the PAX8 protein in the neuronal cells of the insomnia model rats was inhibited and downregulated significantly at 72 h after addition of miR-101a mimics compared with that in the scramble added group (P<0.01). The levels of the interleukins IL-1, IL-2, and IL-6 and the tumor necrosis factor-alpha in the hypothalamus, hippocampus, and prefrontal cortex decreased significantly compared with those in the blank control group (P<0.05). The levels of noradrenaline, dopamine, and glutamic decreased significantly following warm acupuncture or western medicine treatment (P<0.05). In conclusion, this study demonstrates that the upregulation of miR-101a in the rats treated with warm acupuncture is directly associated with PAX8 regulation."

Evidence Sentence: The results of luciferase in the 293T cells indicated that pGL3M-MUT-PAX8-3'UTR and pGL3M-WT-PAX8-3'UTR in the negative control group had no significant changes compared with pGL3M in the blank plasmid group.
Evidence Sentence: Western blotting was used to detect the expression level of PAX8.
Evidence Sentence: The western blotting result indicated that the expression of the PAX8 protein in the neuronal cells of the insomnia model rats was inhibited and downregulated significantly at 72 h after addition of miR-101a mimics compared with that in the scramble added group (P<0.01).
Evidence Sentence: In conclusion, this study demonstrates that the upregulation of miR-101a in the rats treated with warm acupuncture is directly associated with PAX8 regulation.
Evidence Sentence: miR-101a inhibits the expression of the PAX8 protein
Evidence Sentence: The results of western blotting indicated that the expression of the PAX8 protein in the neuronal cells of the insomnia model rats cultured in vitro with miR-101a mimics added was inhibited and downregulated significantly (P<0.01) compared to the expression of the PAX8 protein in the neuronal cells of the insomnia model rats cultured in vitro with scramble control (Fig.
Evidence Sentence: This indicated that miR-101a can bind to the specific sequence in the WT-PAX8-3'UTR promoter and change the specific sequence of the promoter whereas miR-101a does not function (Fig.