Evidence Details for Pcna
PMID Title Journal Year Abstract
23304201 Therapeutic Effects of Acupuncture through Enhancement of Functional Angiogenesis and Granulogenesis in Rat Wound Healing. Evid Based Complement Alternat Med. 2012;2012:464586. doi: 10.1155/2012/464586. Epub 2012 Dec 10. 2012 Acupuncture regulates inflammation process and growth factors by increasing blood circulation in affected areas. In this study, we examined whether acupuncture has an effect on wound healing in injured rat. Rats were assigned randomly into two groups: control group and acupuncture group. Acupuncture treatment was carried out at 8 sites around the wounded area. We analyzed the wound area, inflammatory cytokines, proliferation of resident cells, and angiogenesis and induction of extracelluar matrix remodeling. At 7 days after-wounding the wound size in acupuncture-treat group was decreased more significantly compared to control group. In addition, the protein levels of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) were significantly decreased compared to the control at 2 and 7 days post-wounding. Also, we analyzed newly generated cells by performing immunostaining for PCNA and using several phenotype markers such as CD-31, alpha-SMA, and collagen type I. In acupuncture-treated group, PCNA-positive cell was increased and PCNA labeled CD-31-positive vessels, alpha-SMA- and collagen type I-positive fibroblastic cells, were increased compared to the control group at 7 days post-wounding. These results suggest that acupuncture may improve wound healing through decreasing pro-inflammatory response, increasing cell proliferation and angiogenesis, and inducing extracellular matrix remodeling."

Evidence Sentence: Also, we analyzed newly generated cells by performing immunostaining for PCNA and using several phenotype markers such as CD-31, alpha-SMA, and collagen type I. In acupuncture-treated group, PCNA-positive cell was increased and PCNA labeled CD-31-positive vessels, alpha-SMA- and collagen type I-positive fibroblastic cells, were increased compared to the control group at 7 days post-wounding.
Evidence Sentence: To investigate whether acupuncture treatment improved newly generated cell during wound healing, the number of proliferating cells were determined by anti-PCNA antibody.
Evidence Sentence: In acupuncture-treated group, PCNA labeled CD-31-positive cells were increased compare to the control group in the wound area (172 +- 9.6 versus 118 +- 18.2, P < 0.05) (Figure 5(g)).
Evidence Sentence: We examine whether acupuncture could promote the induction of extracellular matrix remodeling by immunostaining for PCNA and several phenotype markers including alpha-SMA and collagen type I in the wound area.
Evidence Sentence: In acupuncture-treated group at 7 days post-wounding, the PCNA labeled alpha-SMA-positive cells were significantly increased compared to the control (169 +- 33 versus 129 +- 26, P < 0.05) (Figure 6(g)).
Evidence Sentence: Also, PCNA labeled collagen type I-positive cells were significantly increased compared to the control (93 +- 8 versus 76 +- 14, P < 0.05) (Figure 7).