Evidence Details for Pparg
PMID Title Journal Year Abstract
27179524 Effect of Maternal Electroacupuncture on Perinatal Nicotine Exposure-Induced Lung Phenotype in Offspring. Lung. 2016 Aug;194(4):535-46. doi: 10.1007/s00408-016-9899-7. Epub 2016 May 14. 2016 Aug INTRODUCTION: Pregnant women exposed to tobacco smoke predispose the offspring to many adverse consequences including an altered lung development and function. There is no effective therapeutic intervention to block the effects of smoke exposure on the developing lung. Clinical and animal studies demonstrate that acupuncture can modulate a variety of pathophysiological processes, including those involving the respiratory system; however, whether acupuncture affects the lung damage caused by perinatal smoke exposure is not known. METHODS: To determine the effect of acupuncture on perinatal nicotine exposure on the developing lung, pregnant rat dams were administered (1) saline, (2) nicotine, or (3) nicotine + electroacupuncture (EA). Nicotine was administered (1 mg/kg subcutaneously) once a day and EA was applied to both ""Zusanli"" (ST 36) points. Both interventions were administered from gestational day 6 to postnatal day 21 (PND21), following which pups were sacrificed. Lungs, blood, and brain were collected to examine markers of lung injury, repair, and hypothalamic pituitary adrenal (HPA) axis. RESULTS: Concomitant EA application blocked nicotine-induced changes in lung morphology, lung peroxisome proliferator-activated receptor gamma and wingless-int signaling, two key lung developmental signaling pathways, hypothalamic pituitary adrenal axis (hypothalamic corticotropic releasing hormone and lung glucocorticoid receptor levels), and plasma beta-endorphin levels. CONCLUSIONS: Electroacupuncture blocks the nicotine-induced changes in lung developmental signaling pathways and the resultant myogenic lung phenotype, known to be present in the affected offspring. We conclude that EA is a promising novel intervention against the smoke exposed lung damage to the developing lung."

Evidence Sentence: Concomitant EA application blocked nicotine-induced changes in lung morphology, lung Peroxisome Proliferator-Activated Receptor gamma and Wingless-int signaling, two key lung developmental signaling pathways, hypothalamic pituitary adrenal axis (hypothalamic corticotropic releasing hormone and lung glucocorticoid receptor levels), and plasma beta-endorphin levels.
Evidence Sentence: Initially, using lung morphometry, lung PPARgamma and beta-catenin, and BAL SP-A and SP-B protein levels, as markers of lung injury and repair, two subgroups of animals (n=3-4 for each) were used to assess the effect of sham acupuncture (acupuncture using non-acupoints) and EA alone (without nicotine) on lung development,.
Evidence Sentence: Effect of Electroacupuncture on Perinatal Nicotine-Induced Changes in Lung PPARgamma and Wnt signaling
Evidence Sentence: Since the perinatal nicotine-induced lung phenotype has been shown to at least partially result from imbalanced PPARgamma and Wnt signaling, we next probed for key markers of these signaling pathways.
Evidence Sentence: Consistent with the previously published data, perinatal nicotine exposure resulted in decreased PPARgamma signaling, as indicated by decreased PPARgamma (Western analysis and immunostaining) and its downstream target adipocyte differentiation-related protein (ADRP) (Western analysis) levels, and blockage of these changes by the concomitant EA treatment (Fig.
Evidence Sentence: The blockage of nicotine-induced changes in whole lung PPARgamma and beta-catenin levels was also supported by ELISA (Fig.
Evidence Sentence: In fact, as indicated by ELISA, EA not only resulted in blockage of the nicotine-induced decrease in whole lung PPARgamma levels, but resulted in significantly higher levels than the nicotine exposed group.