Evidence Details for Raf1
| PMID | Title | Journal | Year | Abstract |
|---|---|---|---|---|
| 23970928 | Moxibustion Inhibits the ERK Signaling Pathway and Intestinal Fibrosis in Rats with Crohn's Disease. | Evid Based Complement Alternat Med. 2013;2013:198282. doi: 10.1155/2013/198282. Epub 2013 Jul 22. | 2013 | Intestinal fibrosis is the main pathological process in Crohn's disease (CD); acupuncture and moxibustion can inhibit the process of fibrosis in CD rats, but the regulatory mechanism remains unknown. The present study observed the effect of moxibustion on the extracellular signal-regulated kinase (ERK) signaling pathway in the CD rat. The result shows that the phosphorylation of the Ras, Raf-1, MEK-1, and ERK-1/2 proteins and the expression of the corresponding mRNAs in the colon tissue of CD rat were significantly higher than the normal control group. Both treatments with mild moxibustion and with herb-separated moxibustion significantly reduced the expression of the Ras, Raf-1, MEK-1, and ERK-1/2 proteins and Ras and Raf-1 mRNA. MEK-1 and ERK-1/2 mRNA expression in each treatment group showed a downward trend, and the ERK-1/2 mRNA levels were significantly lower in the mild moxibustion group. It indicates that Ras, Raf-1, MEK-1, and ERK-1/2 are involved in the process of intestinal fibrosis in CD rats. Moxibustion can downregulate the abnormal expression of colonic Ras, Raf-1, MEK-1, and ERK-1/2 protein and mRNA levels in CD intestinal fibrosis in rats. Moxibustion may play a role in the treatment of CD intestinal fibrosis by regulating ERK signaling pathway." |
| Evidence Sentence: | The result shows that the phosphorylation of the Ras, Raf-1, MEK-1, and ERK-1/2 proteins and the expression of the corresponding mRNAs in the colon tissue of CD rat were significantly higher than the normal control group. |
| Evidence Sentence: | Both treatments with mild moxibustion and with herb-separated moxibustion significantly reduced the expression of the Ras, Raf-1, MEK-1, and ERK-1/2 proteins and Ras and Raf-1 mRNA. |
| Evidence Sentence: | It indicates that Ras, Raf-1, MEK-1, and ERK-1/2 are involved in the process of intestinal fibrosis in CD rats. |
| Evidence Sentence: | Moxibustion can downregulate the abnormal expression of colonic Ras, Raf-1, MEK-1, and ERK-1/2 protein and mRNA levels in CD intestinal fibrosis in rats. |
| Evidence Sentence: | The Raf-1 Expression in the Colonic Mucosa of Rat |
| Evidence Sentence: | As shown in Figures 6 and 7, the Raf-1 protein expression in the colonic tissue of the MC group was significantly higher than in the NC group (P < 0.01) and that the Raf-1 protein expression in the colon was reduced to varying degrees after acupuncture treatment (P < 0.05 or P < 0.05). |
| Evidence Sentence: | The results in Figure 8 show that the Raf-1 mRNA levels in the MC group were significantly higher than in the NC group (P < 0.01) and that MM and HSM treatments significantly reduced rat colon Raf-1 mRNA levels (P < 0.05), whereas EA treatment did not significantly affect rat colon Raf-1 mRNA levels. |