Evidence Details for Rbfox3
PMID Title Journal Year Abstract
34804173 Scalp Acupuncture and Treadmill Training Inhibits Neuronal Apoptosis through Activating cIAP1 in Cerebral Ischemia Rats. Evid Based Complement Alternat Med. 2021 Nov 12;2021:1418616. doi: 10.1155/2021/1418616. eCollection 2021. 2021 Stroke is the leading cause of long-term disability in developed countries. Multitudinous evidence suggests that treadmill training treatment is beneficial for balance and stroke rehabilitation; however, the need for stroke therapy remains unmet. In the present study, a cerebral ischemia rat model was established by permanent middle cerebral artery occlusion (pMCAO) to explore the therapeutic effect and mechanism of scalp acupuncture combined with treadmill training on ischemic stroke. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and neuronal nuclear protein (NeuN) double staining and cellular inhibitor of apoptosis protein-1 (cIAP1) and NeuN immunofluorescence double staining were used to detect the short-term and long-term neuroprotective effects of scalp acupuncture combined with treadmill training on pMCAO rats. In addition, the antiapoptotic effect of the combined treatment was evaluated in pMCAO rats transfected with cIAP1 shRNA. Western blotting was used to detect the relative protein expression in the caspase-8/-9/-3 activation pathway downstream of cIAP1 to further clarify its regulatory mechanism. Our results showed that scalp acupuncture combined with treadmill training successfully achieved short-term and long-term functional improvement within 14 days after stroke, significantly inhibited neuronal apoptosis, and upregulated the expression of cIAP1 protein in the ischemic penumbra area of the ischemic brain. However, no significant functional improvement and antiapoptotic effect were found in pMCAO rats transfected with cIAP1 shRNA. Western blotting results showed that the combined therapy markedly inhibited the activation of the caspase-8/-9/-3 pathway. These findings indicate that scalp acupuncture combined with treadmill training therapy may serve as a more effective alternative modality in the treatment of ischemic stroke, playing an antiapoptotic role by upregulating the expression of cIAP1 and inhibiting the activation of the caspase-8/-9/-3 pathway."

Evidence Sentence: Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and neuronal nuclear protein (NeuN) double staining and cellular inhibitor of apoptosis protein-1 (cIAP1) and NeuN immunofluorescence double staining were used to detect the short-term and long-term neuroprotective effects of scalp acupuncture combined with treadmill training on pMCAO rats.
Evidence Sentence: NeuN is a specific protein expressed in the nucleus and cytoplasm of most neurons in the nervous system.
Evidence Sentence: Limited numbers of TUNEL and NeuN positive neurons were observed in the sham operation group, while markedly more TUNEL and NeuN positive neurons were found in the model group on the 3rd, 7th, and 14th days (P < 0.05).
Evidence Sentence: SA, TT, and SA-T treatments significantly decreased the number of TUNEL and NeuN positive neurons in pMCAO rats (P < 0.05), and SA-T showed the highest antiapoptotic effect.
Evidence Sentence: The double immunofluorescence staining results showed that multitudinous cIAP1 and NeuN positive neurons existed in the cortex penumbra of the ischemic brain in the sham-operated group (Figure 4).
Evidence Sentence: Compared with the sham-operated group, the fluorescence intensity of cIAP1 and NeuN proteins and the number of cIAP1 and NeuN positive neurons were markedly reduced on the 3rd, 7th, and 14th days in the model group (P < 0.05).
Evidence Sentence: SA, TT, and SA-T significantly increased the fluorescence intensity of cIAP1, NeuN, and the number of cIAP1 and NeuN positive neurons in pMCAO rats (P < 0.05), with SA-T showing the highest effect.
Evidence Sentence: Effect of SA-T on Neuronal Apoptosis and NeuN Expression in the cIAP1 shRNA Interference pMCAO Model
Evidence Sentence: The same results were also confirmed by Western blotting, which showed that the expression level of NeuN protein in the SA-T and SA-T + Lv cIAP1 NC group significantly increased when compared with the model group (P < 0.05) (Figure 7(b)).
Evidence Sentence: In addition, Western blotting results showed that the NeuN protein expression level in the SA-T + Lv cIAP1 group was significantly lower than that in the SA-T group (P < 0.05).
Evidence Sentence: Even on the 3rd and 7th days, the expression of NeuN protein in the SA-T + Lv cIAP1 group was significantly lower than that in the model group (P < 0.05).