Evidence Details for Sigmar1
PMID Title Journal Year Abstract
33384922 The effect of acupuncture stimulation on alleviating emotional changes due to acute alcohol administration and the possibility of sigma(1) receptor involvement. Integr Med Res. 2021 Jun;10(2):100497. doi: 10.1016/j.imr.2020.100497. Epub 2020 Aug 12. 2021 Jun BACKGROUND: Most ETOH addiction preclinical studies have focused on the rewards of chronic ETOH self-administration or the ETOH reinstatement model. Acute ETOH administration studies are scarce despite the potential of ETOH to cause sedation, intoxication and reduced acute functional tolerance. Here, we established a rat model of acute ETOH administration induced by an intraperitoneal injection of 1 g/kg ethanol and assessed the similarities in physiological and behavioral effects between acupuncture and Sigma1 R antagonists. METHODS: Male Wistar rats (300-330 g) received pretreatment with (1) saline injection, (2) saline + mechanical stimulation using a mechanical acupuncture instrument (MAI) for acupuncture at the Shenmen (HT7), (3) ETOH (1 g/kg) injection, (4) ETOH + HT7, or (5) the selective sigma(1) R antagonist BD 1047 (3, 10, or 30 mg/kg, intraperitoneal (IP) injection). ETOH (1 g/kg) or saline was IP injected after 10 min. Then, ETOH-induced immobility was evaluated in an open field arena, ultrasonic vocalizations (USVs) indicating ethanol-induced emotional changes were recorded in a recording chamber, and the rats were sacrificed for the analysis of protein levels of sigma(1) R in several regions of the brain. RESULTS: Acute ethanol exposure increased the immobile time, 22-kHz USVs, and protein levels of sigma(1) R in the ventral tegmental area (VTA). However, pretreatment with acupuncture at HT7 induced recovery of immobile time, reduced 22-kHz USVs, and regulated the protein levels of sigma1 R in the VTA. These effects have similarities with IP injection of BD 1047 (10 mg/kg). CONCLUSION: This study showed that acupuncture at HT7 regulates immobility and 22-kHz USVs via Sigma(1) R in the VTA upon acute ETOH exposure."

Evidence Sentence: Then, ETOH-induced immobility was evaluated in an open field arena, ultrasonic vocalizations (USVs) indicating ethanol-induced emotional changes were recorded in a recording chamber, and the rats were sacrificed for the analysis of protein levels of sigma1 R in several regions of the brain.
Evidence Sentence: Acute ethanol exposure increased the immobile time, 22-kHz USVs, and protein levels of sigma1 R in the ventral tegmental area (VTA).
Evidence Sentence: However, pretreatment with acupuncture at HT7 induced recovery of immobile time, reduced 22-kHz USVs, and regulated the protein levels of sigma1 R in the VTA.
Evidence Sentence: This study showed that acupuncture at HT7 regulates immobility and 22-kHz USVs via Sigma1 R in the VTA upon acute ETOH exposure.
Evidence Sentence: We measured the level of sigma1 R expression in each region of the rat brain when immobility and 22-kHz emissions were increased due to ETOH administration.
Evidence Sentence: Compared with saline treatment, acute injection with ETOH (1 g/kg, IP) significantly increased the protein levels of Sigma1 R in the VTA but not in the PFC, the NAc, the Hip, or the Amg compared with saline (Fig.
Evidence Sentence: Pretreatment with acupuncture at HT7 or IP injection of BD 1047 (10 mg/kg) reduced ETOH-induced increases in the protein levels of Sigma1 R in the VTA (Fig.
Evidence Sentence: Here, we established a rat model of acute ETOH administration induced by an intraperitoneal injection of 1 g/kg ethanol and assessed the similarities in physiological and behavioral effects between acupuncture and Sigma1 R antagonists.
Evidence Sentence: Male Wistar rats (300-330 g) received pretreatment with (1) saline injection, (2) saline + mechanical stimulation using a mechanical acupuncture instrument (MAI) for acupuncture at the Shenmen (HT7), (3) ETOH (1 g/kg) injection, (4) ETOH + HT7, or (5) the selective sigma1 R antagonist BD 1047 (3, 10, or 30 mg/kg, intraperitoneal (IP) injection).
Evidence Sentence: The effect of acupuncture stimulation on alleviating emotional changes due to acute alcohol administration and the possibility of sigma1 receptor involvement