Evidence Details for Sirt1
PMID Title Journal Year Abstract
35003293 Acupuncture Delays Cartilage Degeneration through Upregulating SIRT1 Expression in Rats with Osteoarthritis. Evid Based Complement Alternat Med. 2021 Dec 31;2021:2470182. doi: 10.1155/2021/2470182. eCollection 2021. 2021 Silent mating type information regulation 2 homolog 1 (SIRT1) has been reported to inhibit osteoarthritic gene expression in chondrocytes. Here, efforts in this study were made to unveil the specific role of SIRT1 in the therapy of acupuncture on cartilage degeneration in osteoarthritis (OA). Specifically, OA was established by the anterior cruciate ligament transection method in the right knee joint of rats, subsequent to which acupuncture was performed on two acupoints. Injection with shSIRT1 sequence-inserted lentiviruses was conducted to investigate the role of SIRT1 in acupuncture-mediated OA. Morphological changes and cell apoptosis in rat OA cartilages were examined by safranin-O staining and terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) assay, respectively. The serum levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-2 in OA rats were assessed by enzyme-linked immunosorbent assay (ELISA). The expressions of SIRT1, cartilage matrix degradation-related proteins (matrix metalloproteinase (MMP)-9 and ADAMTS5), NF-kappaB signaling-related markers (p-p65/p65 and p-IkappaBalpha/IkappaBalpha), and cartilage matrix synthesis-related proteins (collagen II and aggrecan) in the OA cartilage were analyzed by western blot. As a result, acupuncture counteracted OA-associated upregulation of TNF-alpha, IL-2, cartilage matrix degradation-related proteins, and NF-kappaB signaling-related markers, morphological damage, apoptosis, SIRT1 downregulation, and loss of cartilage matrix synthesis-related proteins in rat articular cartilages. SIRT1 silencing reversed acupuncture-induced counteractive effects on the aforementioned OA-associated phenomena (except apoptosis, the experiment regarding which under SIRT1 silencing was not performed). Collectively, acupuncture inhibited chondrocyte apoptosis, inflammation, NF-kappaB signaling activation, and cartilage matrix degradation by upregulating SIRT1 expression to delay OA-associated cartilage degeneration."

Evidence Sentence: Acupuncture Delays Cartilage Degeneration through Upregulating SIRT1 Expression in Rats with Osteoarthritis
Evidence Sentence: Silent mating type information regulation 2 homolog 1 (SIRT1) has been reported to inhibit osteoarthritic gene expression in chondrocytes.
Evidence Sentence: Here, efforts in this study were made to unveil the specific role of SIRT1 in the therapy of acupuncture on cartilage degeneration in osteoarthritis (OA).
Evidence Sentence: Injection with shSIRT1 sequence-inserted lentiviruses was conducted to investigate the role of SIRT1 in acupuncture-mediated OA.
Evidence Sentence: The expressions of SIRT1, cartilage matrix degradation-related proteins (matrix metalloproteinase (MMP)-9 and ADAMTS5), NF-kappaB signaling-related markers (p-p65/p65 and p-IkappaBalpha/IkappaBalpha), and cartilage matrix synthesis-related proteins (collagen II and aggrecan) in the OA cartilage were analyzed by western blot.
Evidence Sentence: As a result, acupuncture counteracted OA-associated upregulation of TNF-alpha, IL-2, cartilage matrix degradation-related proteins, and NF-kappaB signaling-related markers, morphological damage, apoptosis, SIRT1 downregulation, and loss of cartilage matrix synthesis-related proteins in rat articular cartilages.
Evidence Sentence: SIRT1 silencing reversed acupuncture-induced counteractive effects on the aforementioned OA-associated phenomena (except apoptosis, the experiment regarding which under SIRT1 silencing was not performed).
Evidence Sentence: Collectively, acupuncture inhibited chondrocyte apoptosis, inflammation, NF-kappaB signaling activation, and cartilage matrix degradation by upregulating SIRT1 expression to delay OA-associated cartilage degeneration.
Evidence Sentence: Acupuncture Reversed OA-Associated Regulation of the Expressions of SIRT1, MMP-9, ADAMTS-5, and the NF-kappaB Signaling Pathway in Rat Articular Cartilages
Evidence Sentence: A previous study has documented that overexpression of SIRT1 reversed IL-1beta-induced upregulation of osteoarthritic genes including MMP-9 and ADAMTS5 in human chondrocytes.
Evidence Sentence: By western blot, we found that SIRT1 expression level in rat articular cartilages was lower in the Model group than that in the Sham group (P < 0.001), while MMP-9 and ADAMTS5 expression levels of rat articular cartilages in the Model group exceeded those in the Sham group (P < 0.01, P < 0.001; Figure 2(a) and 2(b)).
Evidence Sentence: Treatment with acupuncture reversed those OA-associated downregulation of SIRT1 and upregulations of MMP-9 and ADAMTS-5 (P < 0.01, P < 0.001; Figures 2(a) and 2(b)).
Evidence Sentence: Acupuncture Inhibited OA-Associated Inflammation, the NF-kappaB Signaling Pathway, and ECM Degradation through Upregulating SIRT1 Expression in Rat Articular Cartilages
Evidence Sentence: To investigate SIRT1-associated influences in OA-related gene expressions, we injected lentivirus vectors inserted with shSIRT1 into the affected articular cavity of OA rats.
Evidence Sentence: Also, western blot proved that MMP-9 and ADAMTS5 levels were augmented, and SIRT1 level was dwindled in the Model + shSIRT1 group (P < 0.001; Figures 3(c)-3(d)).