| Evidence Sentence: |
Western blotting techniques were utilised to assay the degree of protein expression of NeuN, sequestosome 1 (p62), nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1 (Keap1), glutathione peroxidase 4 (GPX4) and ferritin heavy chain 1 (FTH1). |
| Evidence Sentence: |
The pathways responsible for these effects may encompass amplified p62, Nrf2, GPX4 and FTH1 expression, together with decreased Keap1 expression. |
| Evidence Sentence: |
It can be surmised that intervention with SA enhanced recovery after ICH by triggering the antioxidant pathway, p62/Keap1/Nrf2, and causing FTH1 and GPX4 upregulation, factors that participate in diminishing excess iron and thus in mitigating lipid peroxidation insults arising from ferroptosis following ICH. |
| Evidence Sentence: |
SA Treatment Promotes Nrf2 Transcription by Enhancing the Interaction Between p62 and Keap1 |
| Evidence Sentence: |
), the substrate adaptor p62 protein, also referred to as sequestosome 1, has an immediate impact on Nrf2 expression by attaching to Keap1. |
| Evidence Sentence: |
In order to further appreciate the pathways involved in the upregulation of Nrf2 following ICH, Western blot was utilised to assay the degree of expression of p62 and Keap1 proteins (Fig. |
| Evidence Sentence: |
In cerebral samples from the rats with ICH, p62 expression was elevated and Keap1 protein expression was diminished. |
| Evidence Sentence: |
Furthermore, in contrast to the ICH cohort, rats receiving SA therapy demonstrated heightened expression of p62 protein and reduced Keap1 protein expression on days 1 and 3, respectively, following ICH (Fig. |
| Evidence Sentence: |
The possibility of p62 attaching to Keap1 so as dislodge Nrf2, to suppress Nrf2 breakdown and to promote nuclear accretion, was then explored. |
| Evidence Sentence: |
Western blot data revealed a marked rise in Nrf2 titres in the immediate phase concomitant with the rise and fall in p62 and Keap1 concentrations, respectively (Fig. |
| Evidence Sentence: |
All these findings imply that the engagement of p62 and Keap1 underlies the nuclear amassment of Nrf2 in ferroptosis, and that therapy with SA may augment Nrf2 transcription by promoting the association between p62 and Keap1. |
| Evidence Sentence: |
Western blotting techniques were utilised to assay the degree of protein expression of NeuN, sequestosome 1 (p62), nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1 (Keap1), glutathione peroxidase 4 (GPX4) and ferritin heavy chain 1 (FTH1). |
| Evidence Sentence: |
), the substrate adaptor p62 protein, also referred to as sequestosome 1, has an immediate impact on Nrf2 expression by attaching to Keap1. |
| Evidence Sentence: |
Scalp Acupuncture Protects Against Neuronal Ferroptosis by Activating The p62-Keap1-Nrf2 Pathway in Rat Models of Intracranial Haemorrhage |
